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Questions related from Lucian Miles
I incubated HRECs with RIG012 at a 1% dilution (1 μL of RIG012 per mL of culture medium) for 24 hours; this concentration, hereafter referred to as 1x, has been previously shown to exert...
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I performed co-immunoprecipitation (co-IP) with mouse anti-GFP antibody and mouse anti-myc antibody. Non-specific bands appeared in all samples in the co-IP experiment. Non-specific bands were...
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I am culturing Vero cells and have noticed large vacuoles in them. Does this indicate aging? What other signs of aging are there in animal cell cultures? [Questions are selected from...
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Recently, we are conducting polarization experiments on Raw264.7 macrophages. It is known that Raw264.7 is a classic mouse macrophage cell line that can be induced to polarize to M1/M2...
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When I used the β-Galactosidase staining kit (HY-K1089, MCE) for cell senescence detection, the expected positive signal (such as blue particles) did not appear at all. I used induced senescence...
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Will vortexing cause protein structure destruction or aggregation (such as denaturation and polymer formation)? Do different types of recombinant proteins (such as intracellular expression vs....
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I am studying antibody-drug conjugate (ADC) related technologies and encountered VcMMAE molecules. It is known that VcMMAE is composed of a linker (Vc) and a cytotoxin (MMAE), but how to...
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My plasmid carries the Neo resistance gene (encoding aminoglycoside phosphotransferase). After transfecting mammalian cell lines, can I use Neomycin sulfate (HY-B0470, MCE product) to screen...
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I recently used MCE's Basement Membrane Matrix (HY-K6002) to conduct cell tube formation experiments. I would like to ask about the criteria for judging the tube formation time. I have...
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EdU, as a thymine analog, can be incorporated into newly synthesized DNA chains during the DNA synthesis phase (S phase). Its detection relies on the Cu (I)-catalyzed azide-alkyne cycloaddition...
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I just started to do THP-1 differentiation macrophage experiment recently. I found several versions of the protocol but the details are not consistent. I want to ask for everyone's experience. It...
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When working non-stop in the lab, colleagues like to chat about what’s happening in the lab and share their frustrations with each other. However, some people are proud of their lack of sleep,...
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