102 Questions 313 Answers 0 Followers
Questions related from Lucian Miles
I plan to use Aβ25-35 to treat HT22 cells for establishing an Alzheimer’s disease model. Since oligomers are generally more toxic, they may be more suitable for cell modeling. I dissolved the...
4 weeks ago 7,999 1 View
I am planning to generate cardiac spheroids using induced pluripotent stem cells (iPSCs). However, I need some guidance in selecting the most suitable forms for my experiment. 1. CHIR-99021...
12 August 2025 1,068 1 View
Few questions regarding the use of Mdivi-1 for mitochondrial autophagy inhibition in C57BL/6 mice: I plan to use Mdivi-1 to inhibit mitochondrial autophagy in C57BL/6 mice, but I couldn’t find...
11 August 2025 4,516 1 View
I want to perform mouse colon cell organoid experiments. Should I purchase Ruxolitinib (HY-50856) or Ruxolitinib phosphate (HY-50858)? Many papers do not clearly indicate which form they use,...
08 August 2025 2,123 3 View
I incubated HRECs with RIG012 at a 1% dilution (1 μL of RIG012 per mL of culture medium) for 24 hours; this concentration, hereafter referred to as 1x, has been previously shown to exert...
31 July 2025 8,557 1 View
The markers on the far left are 180 kDa, 130 kDa, 100 kDa, and 75 kDa. The three lanes are control group 1 and treatment group 2, respectively. Each group is separated by a marker. The primary...
31 July 2025 8,606 1 View
I've been using the Bcl-XL Antibody (HY-P80030, from MCE) for my experiments, and I've noticed that the resulting Bcl-XL band appears as a double band. I'm curious why this might be...
31 July 2025 6,278 1 View
I performed co-immunoprecipitation (co-IP) with mouse anti-GFP antibody and mouse anti-myc antibody. Non-specific bands appeared in all samples in the co-IP experiment. Non-specific bands were...
28 July 2025 5,499 1 View
I'm curious about the key differences between SYBR Green II and SYBR Green I Chloride—could you explain their main distinctions in terms of application scenarios or binding properties?...
28 July 2025 5,594 1 View
Are there any fixation, staining, and separation protocols for cells cultured in Matrigel? It would be best if it is for Matrigel of different thicknesses. [Questions are selected from...
28 July 2025 5,755 1 View
I have been using Basement Membrane Matrix Gel to do cell invasion experiments with transfected cells, and now I want to add a drug X (X can promote cell death) to observe its effect on invasion....
28 July 2025 991 2 View
1. After receiving the dye, store it at -20 ℃. When using it, dissolve 100 μg MitoSOX Red with 26 μL DMSO. Dissolve it at 4 ℃ each time you use it, and prepare it into a working solution at a...
25 July 2025 4,694 1 View
After inducing THP- cells with PMA 200ng/ml for 24 hours, I took photos of the cells at 48 hours, 72 hours, 80 hours, and 96 hours. At 96 hours, the bright areas inside the cells seemed to have...
24 July 2025 4,680 2 View
I am studying PBMC cells through CD3/CD28 co-stimulation, and adding Acetylcorynoline (10, 5, 2.5, 1.25) μM at the same time. The reference is PMID: 31724152. After the first treatment of PBMC...
24 July 2025 3,033 2 View
My cells are primary human aortic valve cells, treated with ATF3 Inducer 1 (CAS No.: 3038756-30-5). Culture conditions: cultured in DMEM high glucose medium and osteogenic medium (OM) for two...
24 July 2025 9,133 1 View
I am culturing Vero cells and have noticed large vacuoles in them. Does this indicate aging? What other signs of aging are there in animal cell cultures? [Questions are selected from...
11 July 2025 4,383 3 View
I am planning to test the effects of different drug concentrations on oral squamous cell carcinoma (OSCC) cells using the MTT assay. Can I culture the cells in 6-well plates and then trypsinize...
11 July 2025 7,188 5 View
I always produce large cell pgcc when I do cell transfection. Is there any way to avoid the production of pgcc during transfection? [Questions are selected from MedChemExpress (MCE) customer...
11 July 2025 7,662 1 View
Do you have any MPO inhibitors that you think are useful? Especially inhibitors with good effects and high stability? I know that there are some commonly used ones, such as Verdiperstat...
01 July 2025 4,336 0 View
Recently, I tried to use the Myhc-α(334–352) peptide to induce an autoimmune myocarditis model in mice. I would like to ask about the concentration, dosage and dosage regimen of this peptide~...
01 July 2025 3,256 1 View
Hello, everyone! I would like to ask about the specific steps for PKH26 labeling cells. Currently, I follow the instructions of the kit but the effect is not very good. I would like to confirm:...
01 July 2025 7,759 1 View
When using GlyRS-IN-1 to induce diabetic mouse model for a long time, if gavage is used (8 weeks, 3 times a week), is there a recommended dosage range? Background supplement: Recently, attempts...
25 June 2025 227 1 View
Recently, in fluorescence microscopy observations, it was found that cell lines induced by Doxycycline (HY-N0565, MCE) showed blue fluorescence signals. It is not clear whether it is the...
25 June 2025 7,012 1 View
I am currently planning to conduct gene silencing studies in mouse models. I would like to know the in vivo delivery efficiency, off-target effects, and animal experimental validation data of this...
25 June 2025 869 1 View
Recently, we are conducting polarization experiments on Raw264.7 macrophages. It is known that Raw264.7 is a classic mouse macrophage cell line that can be induced to polarize to M1/M2...
24 June 2025 2,355 1 View
In the literature, there are two common PI3K antibodies, 85kDa and 110kDa subtypes (such as p85α and p110γ), which are known to correspond to different subunits (p85 is the regulatory subunit and...
24 June 2025 5,009 1 View
Hello colleagues, It is known that Cisplatin (CDDP) (HY-17394, MCE) is a platinum-containing anti-tumor drug and belongs to a polar complex. Why do you recommend me to use water or DMF as a...
24 June 2025 5,881 1 View
How do you usually set the parameters when using ultrasonic treatment (such as cell disruption, sample dispersion, etc.)? For example, is there an optimization plan for power, time, temperature...
23 June 2025 1,973 2 View
After Tamoxifen (ICI 47699) is dissolved in corn oil, if it is not used immediately in mouse experiments, what are the key points to pay attention to in terms of storage conditions (such as...
23 June 2025 7,481 1 View
Tislelizumab (HY-P99052, MCE) preparation froze after being stored at -20℃ for a week. Will the drug activity be affected in this case? Can it still be used for experiments after thawing? Are...
23 June 2025 5,061 1 View
I would like to ask about a confusion in siRNA experiments (siRNA set bought from MCE): In recent experiments, the target gene mRNA level dropped significantly, but the protein expression did not...
21 June 2025 4,276 1 View
Hello, teachers/colleagues, I would like to ask about the core difference between Fluo-3 and Fluo-3 AM in application: Both are fluorescent probes for calcium ions, but Fluo-3 AM is often...
21 June 2025 9,833 1 View
I would like to ask whether BODIPY 493/503 (HY-W090090, MCE), as a lipid fluorescent dye, is suitable for fixation and permeabilization after staining? It is known that this dye is often used to...
21 June 2025 2,048 1 View
I would like to ask whether JC-1 (CBIC2) is suitable for bacterial staining as a mitochondrial membrane potential probe? It is known that it is often used for eukaryotic cell mitochondrial...
21 June 2025 3,258 1 View
I would like to ask a question about the use of Glycerol (HY-B1659, MCE): The glycerol I purchased is a liquid reagent. Can it be used directly in cell experiments (such as cell freezing,...
21 June 2025 1,656 1 View
I have a question about recombinant protein SPR experiments: After the recombinant protein was pre-enriched recently, the SPR test results were abnormal (such as weak signal/high non-specific...
21 June 2025 9,976 1 View
Does Metformin (1,1-dimethylbiguanide, MCE HY-B0627), which is commonly used in clinical practice, have a distinct taste? Recently, when mice were given drugs by gavage, it was found that the...
19 June 2025 6,197 1 View
Hello, I encountered several unsolvable problems in the invasion experiment: After the matrix gel was plated, the liquid surface was uneven and bubbles always appeared; After crystal violet...
19 June 2025 9,543 1 View
I am conducting cell mitochondrial research. Can TMRE (mitochondrial membrane potential probe, MCE HY-D0985A) be used with MitoSOX Red (mitochondrial superoxide fluorescent probe, MCE HY-D1055)...
19 June 2025 2,288 1 View
When I used the β-Galactosidase staining kit (HY-K1089, MCE) for cell senescence detection, the expected positive signal (such as blue particles) did not appear at all. I used induced senescence...
17 June 2025 8,612 1 View
Hello, everyone, 1. Why do I observe green fluorescence instead of the expected red under a fluorescence microscope when using Nile Red for lipid staining? 2. Can Nile Red be used for lipid...
17 June 2025 841 1 View
I am preparing to do cell senescence staining recently. The fixative in the kit is almost used up, and the lab just happens to have PFA and formalin. The kit instructions say that the fixative is...
16 June 2025 9,233 1 View
In the study of blood-testis barrier permeability, which molecular weight of FITC-Dextran is usually used? When I read the literature before, I saw that the application scenarios of dextran with...
16 June 2025 6,980 1 View
After inducing cell lipid droplets (such as treating liver cells with oleic acid), I tried to dissolve the lipid droplets by lipid extraction or drug treatment, but found that the effect was not...
13 June 2025 8,672 1 View
Will vortexing cause protein structure destruction or aggregation (such as denaturation and polymer formation)? Do different types of recombinant proteins (such as intracellular expression vs....
12 June 2025 306 5 View
When I buy antibodies, I always wonder whether it is necessary to distinguish different dissolution schemes in immunohistochemistry (IHC) and immunofluorescence (IF) applications, and whether it...
12 June 2025 1,033 2 View
I am planning to design an in vivo dosing scheme for Paclitaxel (HY-B0015, MCE). Your technical support recommends using a mixed solvent of 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline to...
12 June 2025 3,105 1 View
I would like to ask about the dissolution and storage of Camptothecin (HY-16560, MCE) in animal experiments: In rodent (such as mouse/rat) experiments, what solvents are usually used to dissolve...
12 June 2025 6,065 2 View
I am studying antibody-drug conjugate (ADC) related technologies and encountered VcMMAE molecules. It is known that VcMMAE is composed of a linker (Vc) and a cytotoxin (MMAE), but how to...
10 June 2025 1,516 1 View
Hello, I would like to ask about the preparation details of the dissolution scheme for animal experiments of Trametinib (HY-10999, MCE): 1. Is 0.5% HPMC (hydroxypropyl methylcellulose) calculated...
10 June 2025 8,345 1 View
I recently bought Protein A/G Magnetic Beads (HY-K0202) from MedChemExpress. How to accurately evaluate the loading capacity of magnetic beads (such as the binding efficiency of antibody-coated...
09 June 2025 129 1 View
What indicators are usually used to confirm that the mouse Parkinson's disease model is successfully established? For example, are there recommended standards for the application process of...
09 June 2025 6,641 1 View
My plasmid carries the Neo resistance gene (encoding aminoglycoside phosphotransferase). After transfecting mammalian cell lines, can I use Neomycin sulfate (HY-B0470, MCE product) to screen...
04 June 2025 4,031 1 View
I have a question about experimental reagents. I want to use human insulin (HY-P0035, MCE product) to treat 3T3-L1 mouse preadipocytes. I want to confirm: 1. Is human insulin active in mouse...
04 June 2025 5,177 2 View
In recent studies on lung cancer immunotherapy, we plan to use Durvalumab (HY-P9919A/HY-P9919, MedChemExpress) to treat A549 cell lines (human non-small cell lung cancer cells). According to...
03 June 2025 5,177 1 View
Hello, researchers. I would like to ask a question about 3-Color Prestained Protein Marker (10-190 kDa) (Cat.No.: HY-K1011; bought from MedChemExpress, MCE): Will the apparent molecular weight of...
03 June 2025 5,166 1 View
I would like to ask a question about the dosage setting of Sparstolonin B (HY-116213, MedChemExpress) in rat acute pneumonia injury model: Recently, I have set a research project on rat acute...
03 June 2025 8,767 1 View
I recently used MCE's Basement Membrane Matrix (HY-K6002) to conduct cell tube formation experiments. I would like to ask about the criteria for judging the tube formation time. I have...
30 May 2025 2,765 1 View
When I looked up the information, I found that it is often used for oligonucleotide probe quenching, but I am not sure about its broad-spectrum applicability. I would like to ask what are the...
30 May 2025 1,630 1 View
I recently used MCE's TO-PRO 1 (HY-D0917, MCE Product) to conduct nucleic acid staining experiments. I would like to ask about the optimal staining concentration of this reagent. [Troubleshooting...
30 May 2025 8,620 1 View
When using Ni-NTA affinity chromatography gel beads (Cat.No.: HY-K0210, bought from MCE) for His tag protein purification, the color of the gel beads was observed to change (for example, turning...
29 May 2025 4,711 1 View
Tribromoethyl alcohol (Cat.No.: HY-B1372) is a commonly used animal anesthetic in laboratories: In addition to the usual protection of wearing gloves and fume hoods, are there any special...
29 May 2025 2,594 1 View
Question about Calcein (HY-D0040, MCE) and Calcein AM (HY-D0041, MCE) . What are the specific differences between the two in terms of molecular structure, cell permeability and fluorescent...
29 May 2025 9,860 2 View
Regarding the differences in the application of RIPA lysis buffer (HY-K1001, MCE Product) and NP-40 lysis buffer (HY-K1002, MCE Product): Based on the existing experimental knowledge, there are...
28 May 2025 8,991 1 View
Confusion about the experimental application of FITC-Dextran molecular weight selection: FITC-labeled dextran has good water solubility and fluorescence stability, and is often used as a tracer...
28 May 2025 9,847 1 View
EdU, as a thymine analog, can be incorporated into newly synthesized DNA chains during the DNA synthesis phase (S phase). Its detection relies on the Cu (I)-catalyzed azide-alkyne cycloaddition...
22 May 2025 9,639 1 View
What is the commonly used working concentration range of Blasticidin S hydrochloride (Cat.NO.: HY-103401, bought from MCE) in cell screening or gene editing experiments? It is known that this...
22 May 2025 1,662 1 View
I plan to conduct animal experiments on cell proliferation in the near future, and I intend to use 5-BrdU (Cat.No.: HY-15910, bought from MCE) as a marker. I would like to ask the following...
22 May 2025 8,996 1 View
After adding RIPA lysis buffer (Cat.No.: HY-K1001, bought from MCE) to cells, white floccules appear. Is this normal? When I used RIPA lysis buffer to treat adherent/suspended cells, I observed...
22 May 2025 6,130 1 View
Recently, when conducting lipid droplet-related histological research, I encountered a technical confusion: We plan to use BODIPY 493/503 (MCE, Cat.NO.: HY-W090090) to stain tissue samples for...
21 May 2025 9,781 1 View
Little talk: I use CCK-8 (Cat.No.: HY-K0301) and the measured values are different each time. What is the reason? What substances will affect the determination of CCK-8? Is the sensitivity the...
21 May 2025 3,007 1 View
How to prepare 0.5% HPMC/0.1% Tween-80 saline solution to dissolve AZD-7648 (Cat.No.: HY-111783, bought from MCE)? Are there any special requirements for the specific operation steps (such as...
20 May 2025 5,939 1 View
I would like to ask two technical questions about EDANS-CO-CH2-CH2-CO-ALERMFLSFP-Dap(DABCYL)OH (Cat.No.: HY-P5326): 1. Background fluorescence characteristics: Does the probe have...
19 May 2025 6,548 1 View
Could you tell me what the technical meanings of "LALA-PG" and "D665" in the name of Anti-Mouse CD28 (LALA-PG) Antibody (D665) (Cat.NO.: HY-P99123A) are? For example, does it involve antibody...
19 May 2025 3,876 1 View
What are the main differences in structural composition, biological activity or application scenarios of lipopolysaccharides (LPS) of 5 serotypes of Lipopolysaccharides, from E. coli O55:B5...
19 May 2025 1,081 1 View
What are the specific steps for using D-luciferin (Luciferin, D-type, bought from MCE; Cat.NO.: HY-12591B) for in vivo imaging? I need to clarify the following key links: Dissolution...
16 May 2025 411 1 View
How to prepare 20% (w/v) HP-β-CD (Cat.NO.: HY-101103, bought from MCE) solution? Please provide the following experience: Solvent selection (whether it is distilled water or a specific...
16 May 2025 3,811 1 View
Regarding the use of Collagen, rat tail (Cat.NO.: HY-NP010, bought from MCE) to promote cell attachment on thin-layer coated culture plates, I would like to ask the following details: 1. What is...
15 May 2025 9,504 1 View
[Ask] When using the HY-K0318 kit (bought from MCE) to detect NO content, I would like to know the specific operating details of the freeze-lysis step: for example, does the sample need to be...
15 May 2025 6,458 1 View
Hello experts/colleagues! I am currently designing a rat disease model experiment and need to screen a reliable myeloperoxidase (MPO) inhibitor for in vivo intervention. I have the following...
14 May 2025 793 1 View
Hello experts/colleagues! I am currently facing a dosage optimization problem when designing a long-term intervention experiment with GlyRS-IN-1 (Cat.NO.: HY-108940, bought from MCE) in diabetic...
14 May 2025 8,574 1 View
Hello everyone! I recently encountered the details of the dissolution and storage of recombinant human IFN-γ protein (Cat.NO.: HY-P7025) in the experimental preparation: 1. Dissolution...
14 May 2025 9,666 1 View
Hello everyone, when exploring the in vitro cell culture system recently, I paid attention to collagen (bought from MCE, Cat.NO.: HY-NP010) as a natural extracellular matrix (ECM) component. Its...
13 May 2025 4,462 2 View
Recently, when I was establishing a cell senescence detection system, I needed to select specific marker dyes for senescent cells for different experimental purposes (such as real-time tracking of...
13 May 2025 3,216 1 View
I just started to do THP-1 differentiation macrophage experiment recently. I found several versions of the protocol but the details are not consistent. I want to ask for everyone's experience. It...
12 May 2025 7,051 1 View
I want to ask for the activity measurement method of BACE1 Protein, Human (HY-P72840). I bought the recombinant protein of MCE. But when I made it myself, the fluorescence signal was very weak....
12 May 2025 7,252 2 View
What are the fundamental differences between Firefly luciferase (Luciferase from Photinus pyralis ) and Renilla luciferase (Luciferase from Renilla reniformis ), and how do these impact their use...
09 May 2025 5,858 1 View
When working non-stop in the lab, colleagues like to chat about what’s happening in the lab and share their frustrations with each other. However, some people are proud of their lack of sleep,...
01 January 1970 6,866 5 View
It's called "lithofacia," which literally means "stone baby." This condition usually occurs during an abdominal pregnancy (an ectopic pregnancy in which the embryo implants in the abdomen): when...
01 January 1970 7,130 0 View
It's must be exhausted. The revision process for the writing and figures was brutal. The reviewers requsted to expand the scope of the study dramatically and then add more writing. At the end, the...
01 January 1970 773 1 View
MedChemExpress (MCE) is excited to exhibit at the American Association for Cancer Research (AACR) Annual Meeting, taking place April 26-30 at McCormick Place Convention Center in Chicago! The...
01 January 1970 7,686 5 View
Today I saw a researcher's "extra" question- what does polymerase smell like? What about elution buffer? Saccharomyces cerevisiae smells like strong sweet wine, what does it smell like? What does...
01 January 1970 1,186 6 View
Remember surgery on a grape? Now we have elephants in cells! Seriously, in the future, instead of removing microplastics from tissues, we will use them to 3D print limbs as cells or other...
01 January 1970 8,106 2 View
Calcium ions (Ca²⁺) play a crucial role in various biological processes, including cell signal transduction, neuronal and muscle electrical activity, apoptosis, drug action mechanisms, immune...
01 January 1970 3,665 1 View
When chatting with our colleagues, we mentioned a misunderstanding: scientists may tend to write very complete papers. Some outside this circle may question: Why do we have to tell a full...
01 January 1970 8,423 1 View
It is widely believed that both low testosterone levels and advanced age are likely to reduce sperm quality—in terms of motility, morphology, and other measures. And poor sperm quality can make...
01 January 1970 4,881 6 View
MedChemExpress (MCE) is participating in the 108th Annual Meeting of the German Society of Pathology (12th-14th June 2025)! https://www.pathology-congress.de/ You can get the DGP Annual...
01 January 1970 8,032 1 View
MCE webinar introduces Teleofection, a cutting-edge two-step process to enhance the formation of biocompatible CaP nanoparticles. Teleofection facilitates the delivery of nucleic acid and...
01 January 1970 2,960 1 View
I'm excited to announce that MedChemExpress (MCE) will be attending the CIMT Annual Meeting 2025-one of the most important gatherings in cancer immunotherapy research! Date: May 12–14,...
01 January 1970 1,707 1 View
Webinar Speaker: Mariano Barbacid, PhD Group Leader of Experimental Oncology Group, Molecular Oncology Program Spanish National Cancer Research Centre (CNIO), Madrid, Spain Pioneer in Cancer...
01 January 1970 855 0 View
Biology is full of fascinating "rabbit holes" - fields that are superficial at first glance, but reveal complex mechanisms, subvert cognition, or show the exquisite design of life. I have...
01 January 1970 2,808 0 View
The first cross-species mechanism of microbial metabolism driving distal tumors is revealed (Nature. 2024 Aug;632(8027):1137-1144.) Gut microbiota carcinogen metabolism causes distal tissue...
01 January 1970 8,380 2 View
