Blasticidin S is commonly used in molecular biology experiments for stable transfection screening of mammalian cells carrying bsr/BSD resistance genes.

The specific application scenarios and concentration specifications are as follows:

1. Stable transfection screening of mammalian cells

As a selective reagent for bsr/BSD genes, the recommended working concentration needs to be optimized according to the cell type, with a typical range of 1-20 μg/mL. This concentration range can effectively inhibit the growth of untransfected cells while ensuring the selective amplification of positive clones.

2. Prevention of cell culture contamination

When used to inhibit contamination by microorganisms such as mycoplasma, it is recommended to maintain culture at a low concentration of 1-20 μg/mL. Note: This concentration range needs to be verified by preliminary experiments to verify cell tolerance and avoid inhibitory effects on the proliferation of target cells.

3. Bacterial selective culture

As an antibiotic screening reagent for prokaryotic cells (such as Escherichia coli), the recommended working concentration is 50-100 μg/mL, and the matching LB culture medium must meet the following requirements:

• Sodium chloride concentration ≤ 90 mM (to avoid hypertonic environment affecting bacterial growth);
• Culture medium pH ≤ 7.0 (to maintain reagent stability and bacterial metabolic activity).

4. Operation points:

All application scenarios require setting up concentration gradient pre-experiments, and optimizing specific parameters in combination with cell/strain tolerance differences; Reagents must be stored at low temperature (-20°C) away from light to maintain activity.

The answer to this question comes from MCE Technical Support.

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