In animal experiments, Camptothecin can be dissolved in a 15% Cremophor EL + 85% saline solution. This laboratory has verified that this solution can effectively prepare a suspension with a concentration of 10 mg/mL (28.71 mM). The specific operations and precautions are as follows:

Preparation process:

First, fully mix Cremophor EL and saline in a volume ratio of 15:85, and then add camptothecin raw materials;

If precipitation or precipitation occurs, ultrasound-assisted dissolution can be used (power and time need to be optimized according to experimental conditions).

Storage recommendations:

This system is a ready-to-use solution. Due to the stability of the solution, it is not recommended to store it. It needs to be used as soon as possible within a short period of time after preparation.

This solution needs to pay attention to the biocompatibility of the solvent system. It is recommended to evaluate the animal's tolerance to Cremophor EL before the experiment.

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Camptothecin (CPT) is a poorly water-soluble compound that requires careful preparation for in vivo studies. Here are the recommended guidelines for its dissolution and storage based on common laboratory practices and MCE product handling standards:

1. Solvent Selection for CPT Dissolution in Animal Experiments

✅ Commonly Used Solvents:

• DMSO (Dimethyl Sulfoxide): Most commonly used solvent due to the high solubility of CPT.
• Ethanol: Sometimes used in combination with other solvents to reduce toxicity.
• Solvent Mixtures: A common mixture is: DMSO : PEG300: Tween 80: Saline (or PBS) in ratios like 1:4:1:4 or 1:2:1:6. This reduces DMSO toxicity and improves injection tolerance in rodents.

⚠️ Toxicity Considerations:

• DMSO: Safe up to 10% for IP injection in rodents, but >10% can be toxic.
• Ethanol: Should be diluted below 5% for systemic use.
• Always use minimal effective concentrations of organic solvents and adjust vehicle composition based on route of administration (IP, IV, PO).

2. Recommended Dissolution Steps

To ensure complete and stable dissolution of CPT:

• Step 1: Dissolve CPT powder in 100% DMSO to create a concentrated stock solution (e.g., 10–20 mM).
• Step 2: Vortex well.
• Step 3: Use ultrasonication (5–10 min) if needed. Do not heat, as CPT is heat-sensitive and may degrade.
• Step 4: Dilute the stock solution with pre-warmed vehicle (e.g., PEG/Tween/saline mix) before injection.

3. Storage of CPT Solutions

📦 Storage Conditions:

• Concentrated DMSO stock: Store at -20°C, protected from light.
• Working solutions (diluted with vehicle): Store at 4°C for short term (≤1 week), preferably fresh before use.

🔒 Light Sensitivity:

• Yes, CPT is light-sensitive. Always wrap vials in foil or store in amber tubes to prevent degradation.

✔️ Stability Verification:

• Before each use, inspect the solution for precipitation or color change.
• If stored >1 week, consider re-testing stability using HPLC or UV-Vis if available, or prepare fresh solution.

If you're using a specific administration route (IP, IV, PO), further formulation adjustment may be needed. Always refer to animal ethics and safety guidelines when preparing dosing solutions.