[Ask] When using the HY-K0318 kit (bought from MCE) to detect NO content, I would like to know the specific operating details of the freeze-lysis step: for example, does the sample need to be pre-cooled?
How to set the temperature and time during lysis?
Is special centrifugation or supernatant treatment required after lysis?
I hope experienced colleagues can share the key points of operation, thank you!
The steps for using Nitric Oxide Detection Kit (HY-K0318) are as follows:
Adherent cells are treated with trypsin or cell scraping and then transferred to a centrifuge tube, and suspended cells are directly transferred to an EP tube.
Then, centrifuge at 4℃, 1000×g for 10 min to collect the cells.
Wash the cells with pre-cooled PBS, centrifuge at 4℃, 1000×g for 10 min, remove the supernatant, and repeat 2-3 times.
The steps for freezing and lysis are as follows:
The answer to this question comes from MCE Technical Support.
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