In the literature, there are two common PI3K antibodies, 85kDa and 110kDa subtypes (such as p85α and p110γ), which are known to correspond to different subunits (p85 is the regulatory subunit and p110 is the catalytic subunit).
Currently, I plan to detect the expression of PI3K in mammalian cell lines. I would like to ask:
Is there a difference in the specific performance of these two subtypes of antibodies in WB?If both subunits exist in the sample, is it necessary to optimize the primary antibody concentration?
Are there any recommended brands or product numbers (such as CST, Abcam, etc.) that work better in similar experiments?
[Troubleshooting Questions are selected from MCE customer consultation emails.]
In the PI3K signaling pathway, p85 and p110 are both core subunits, and their functions have a clear division of labor:
As a regulatory subunit, the p85 subunit mainly mediates the intracellular localization and activity regulation of PI3K;
As a catalytic subunit, the p110 subunit has kinase activity and can catalyze the phosphorylation of phosphatidylinositol (such as PIP2) to generate second messengers (such as PIP3), which is a key functional unit of signal transduction.
The two mediate the activation and conduction of the PI3K signaling pathway in a heterodimer form.
Therefore, the selection of antibodies in WB experiments must be strictly based on the detection target -
- if you focus on the regulatory mechanism of PI3K, you should give priority to p85 antibodies;
- if you focus on catalytic activity or signal transduction function, you need to choose p110 antibodies specifically.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
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