Hello Lucian Miles

You may prevent formation of PGCC by reducing cellular stress. This may be done by

1. Using healthy and actively dividing cells.

2. Maintaining cells at the appropriate confluency (around 40-70%) to ensure proper cell-to-cell contact.

3. You may experiment with different transfection reagents to find which one works best for your cell line and transfection method.

4. Optimizing the incubation time of the transfection complex with cells so that you minimize the duration of exposure to potentially harmful reagents.

5. Avoiding overloading of cells with DNA which can lead to stress and PGCC formation. Try to optimize the DNA concentration.

6. Using high-quality, endotoxin-free DNA is essential for efficient transfection and can indirectly help minimize PGCC formation by reducing stress on the cells.

7. Using cells within an appropriate passage number (lower passage) can contribute to better transfection results and potentially reduce PGCC formation.

By carefully controlling these factors, you can minimize the formation of PGCCs during transfection experiments and obtain more reliable results.

Best,