Yes, the molecular weight of MCE's 3-Color Prestained Protein Marker (10-190 kDa) (Cat.No.: HY-K1011) may be different in different buffers.

HY-K1011 Introduction:

3-Color Prestained Protein Marker (Cat.No.: HY-K1011) is a prestained protein marker (Prestained Protein Marker) that achieves visual labeling through covalent modification of dyes and proteins. 3-Color Prestained Protein Marker (10-190 kDa) consists of 10 prestained proteins with a molecular weight range of 10-190 kDa.

Migration characteristics of HY-K1011:

Generally, the 65 kDa band of 3-Color Prestained Protein Marker is orange, the 23 kDa band is green, and the remaining bands are blue (10, 17, 32, 42, 54, 95, 140, 190 kDa). After SDS-PAGE electrophoresis, clear colored bands can be seen when transferred to PVDF membrane and NC membrane.

However, the electrophoretic migration characteristics of 3-Color Prestained Protein Marker are essentially different from those of natural proteins and are more susceptible to the influence of buffer systems (such as pH value, ionic strength, additive components, etc.).

If you need to estimate the molecular weight of the marker, it is recommended to use the migration spectrum of the marker verified under the same buffer conditions as the experimental system to ensure the accuracy of the molecular weight assessment.

The migration spectrum of the marker for the corresponding buffer can be obtained on the product details page of MCE.

HY-K1011 introduction page:

https://www.medchemexpress.com/inhibitor-kit/3-color-prestained-protein-marker-10-190-kda.html

HY-K1011 Manual:

https://file.medchemexpress.com/inhibitorKitUpload/HY-K1011/MCE-3-Color-Prestained-Protein-Marker-10-190kDa-Manual.pdf

The answer to this question comes from MCE Technical Support.

How to contact us?

• Tel: 609-228-6898
• Fax: 609-228-5909
• E-mail: [email protected]
• Technical Support: [email protected]
• Address: 1 Deer Park Dr, Suite F, Monmouth Junction, NJ 08852, USA