What are the fundamental differences between Firefly luciferase (Luciferase from Photinus pyralis ) and Renilla luciferase (Luciferase from Renilla reniformis ), and how do these impact their use in dual-reporter gene assays or single-protein expression studies?
Firefly luciferase is a protein with a molecular weight of about 61 kD. In the presence of ATP, magnesium ions and oxygen, it can catalyze the oxidation of luciferin to oxyluciferin.
During the oxidation of luciferin, it emits bioluminescence with a wavelength of about 560 nm. It is currently the most commonly used luciferase in in vivo and in vitro detection experiments.
Renilla luciferase is a protein with a molecular weight of about 36 kD. In the presence of oxygen, it can catalyze the oxidation of coelenterazine to coelenteramide.
The reaction process does not require the participation of ATP and magnesium ions. During the oxidation of Coelenterazine (Cat.NO.: HY-18743), it emits bioluminescence with a wavelength of about 480 nm.
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