I am planning to design an in vivo dosing scheme for Paclitaxel (HY-B0015, MCE). Your technical support recommends using a mixed solvent of 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline to dissolve Paclitaxel.
How well is this solvent system tolerated in rodents (e.g., mice/rats)? Is there any literature or experimental data to support this?
Is it possible that the concentration of each component (especially the 10% concentration of DMSO) may induce animal toxicity? Is it necessary to adjust the ratio?
If the drug is administered intraperitoneally/intravenously, is it necessary to pay attention to the injection volume limit or dosing interval?
The laboratory found that the dissolution effect of this system was good in the preliminary experiment, but is concerned about the impact of the solvent on animal health.
[Troubleshooting Questions are selected from MCE customer consultation emails.]
Yes, the dissolution system of 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline is tolerable in rats and mice.
Experiments by MCE customers have confirmed that paclitaxel dissolved in this solution has effective anti-tumor activity in animals;
Published literature (PMID: 34388391) shows that in the GEM spontaneous tumor mouse derived tumor tissue transplantation (GDA) model, the use of 10 mg/kg intraperitoneal injection (ip.), once a week, the dilution of this dissolution solution can significantly inhibit the growth of tumor volume.
The experimental results of the literature show that its inhibitory effect on tumor growth is statistically significant.
Reference: https://pubmed.ncbi.nlm.nih.gov/34388391/
The answer to this question comes from MCE Technical Support.
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