I would like to ask whether BODIPY 493/503 (HY-W090090, MCE), as a lipid fluorescent dye, is suitable for fixation and permeabilization after staining?
It is known that this dye is often used to label lipid droplets in living cells. If immunofluorescence and other operations are required later, will the fixative (such as paraformaldehyde) or permeabilization agent (such as Triton X-100) affect its fluorescent signal?
I hope to share your operating experience or precautions. Thank you!
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BODIPY 493/503 can be used for post-staining fixation.
For specific operations, please refer to the protocol (PMID: 24099295):
This dye is a lipophilic fluorescent dye that can stain the neutral lipid core of fixed cells. It is usually added simultaneously with the primary or secondary antibody after the fixation step, with a final concentration of 1-10 μg/ml and incubated at 37°C for at least 1 hour.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
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