When using GlyRS-IN-1 to induce diabetic mouse model for a long time, if gavage is used (8 weeks, 3 times a week), is there a recommended dosage range?
Background supplement:
Recently, attempts have been made to establish a diabetic model by targeting the GlyRS pathway. Preliminary preliminary experiments have found that different doses of GlyRS-IN-1 (0.5-2 mg/kg) can affect blood glucose levels after short-term administration, but there are differences in long-term toxicity and model stability. Considering the relationship between gavage frequency and metabolic accumulation, I hope to know whether there is a dosage regimen supported by literature or experience, especially in combination with specific data of mouse strains (such as C57BL/6).
Looking forward to sharing guidance!
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Professional explanation on the dosage of GlyRS-IN-1 (HY-108940, MCE) in mouse models:
The setting of dosage is the core design content of the experimental plan and needs to be determined independently by the researcher based on the specific research purpose, model characteristics and pre-experimental data.
Regarding the GlyRS-IN-1 product you consulted, the only public document available is patent document WO 2017066459 A1, with the dosage plan is 0.4 mg/kg (10 μΜ), 2.0 mg/kg (50 uM), 4.0 mg/kg (100 μΜ). But the model involved in this patent is different from the diabetes model you mentioned, so the recommended dosage is not directly referenced.
It is recommended to conduct pre-experimental exploration by setting a dosage gradient (such as high and low dose groups).
If you need to estimate the purchase volume of the product, you can calculate it according to the following formula:
Purchase volume (mg) = Dosage (mg/kg) × Mouse weight (kg) × Number of mice × Total number of dosing
For example: If 4 mg/kg is used for gavage of 20 g (0.02 kg) C57BL/6 mice (10 mice), and the dosing cycle is 8 weeks (3 times per week, a total of 24 times), the required dosage is:
4 mg/kg × 0.02 kg × 10 × 24 = 19.2 mg.
The answer to this question comes from MCE Technical Support.
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