Take a look at our protocol for inducing THP-1 cell differentiation.

(1) THP-1 cells were seeded in a well plate (density of 5 × 10^5 cells/mL), PMA (concentration of 50-200 ng/mL) was added, and the cells were treated for 24 h to induce differentiation.

(2) Polarization of M0 resting macrophages after treating the cells with PMA for 24 h, the differentiated adherent cells were washed twice with serum-free medium and then rested in medium without PMA for 24 h to obtain M0 resting macrophages.

(3) Polarization of M1 classically activated macrophages after treating the cells with PMA for 24 h, the cells were co-cultured with 100 ng/mL LPS and 20 ng/mL IFN-γ for 24 h to obtain classically activated M1 macrophages.

(4) Polarization of M2 alternatively activated macrophages after treating the cells with PMA for 24 h, the cells were co-cultured with 20 ng/mL IL-4 and 20 ng/mL IL-13 for 24 h to obtain alternatively activated M2 macrophages.

The answer to this question comes from MCE Technical Support. How to contact us?

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