Hello everyone! I recently encountered the details of the dissolution and storage of recombinant human IFN-γ protein (Cat.NO.: HY-P7025) in the experimental preparation:

1. Dissolution feasibility

RPMI1640 serum-free medium (without phenol red and additional additives, pH 7.2-7.4) is planned to be used for direct dissolution. Is the buffer system (HEPES/bicarbonate) of this medium suitable for the stable dissolution of the protein? Do we need to pay attention to the potential effects of specific components in the medium (such as inorganic salts, vitamins) on the protein structure?

2. Storage time after dissolution

If the product is dissolved at the recommended concentration (such as 0.1-1 mg/mL), how long is the reasonable storage time recommended for short-term (4℃) and long-term (freezing)? Is it necessary to sterilize through a 0.22μm filter membrane?

3. Stock solution freezing conditions

If the mother solution is directly prepared using the culture medium and aliquoted and frozen at -20℃, what operational details should be paid special attention to?

If you have relevant operation experience or literature reference, please share your suggestions! Looking forward to your reply, thank you!