After inducing cell lipid droplets (such as treating liver cells with oleic acid), I tried to dissolve the lipid droplets by lipid extraction or drug treatment, but found that the effect was not ideal.
I have tried isopropanol and chloroform-methanol systems, but there are many lipid droplets left. I want to know (thanks in advance):
Is there a recommended combination of lipid droplet dissolution reagents or an optimized protocol?
Do I need to pay special attention to the temperature, time or cell treatment conditions in the dissolution step?
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Your question is pretty vague, so I will try to guess what you mean.
First question, can you describe your lipid extraction protocol (presumably) from cells?
Another question is: how do you know, you have nondissolved lipid droplets?
The first thing that comes on my mind is that your cell lysis is deficient. I assume you assume you have not all lipid droplets (or rather lipids in total) dissolved by some aggregates in your sample, which most likely are not nondissolved lipids, but rather some cellular debris.
Therefore the answer for my first question is crucial.
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