Why does the Bcl-XL band produced by the Bcl-XL Antibody (YA588) antibody appear as a double band?

31 July 2025 1 6K Report

I've been using the Bcl-XL Antibody (HY-P80030, from MCE) for my experiments, and I've noticed that the resulting Bcl-XL band appears as a double band. I'm curious why this might be happening—could it be related to post-translational modifications, antibody cross-reactivity, or something else? Would appreciate any insights from fellow researchers who have experience with this antibody or similar observations.

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Lucian Miles

The Bcl-XL gene itself has isoforms, so the presence of two bands is normal. This phenomenon has also been found in other literature (see attached figure).

The answer to this question comes from MCE Technical Support.

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