Regarding the differences in the application of RIPA lysis buffer (HY-K1001, MCE Product) and NP-40 lysis buffer (HY-K1002, MCE Product):
Based on the existing experimental knowledge, there are significant differences in the lysis mechanism between the two:
- RIPA lysis buffer is a strong lysis system. With detergent components such as SDS, it can effectively destroy the cell membrane and nuclear membrane structure. Therefore, it is often used in scenarios where cytoplasmic and nuclear proteins are extracted at the same time (such as transcription factor detection);
- NP-40 lysis buffer is characterized by mild lysis. It only acts on the lipid layer of the cell membrane and can retain the integrity of subcellular structures such as the nucleus. It is more suitable for simple cytoplasmic protein separation (such as cytoplasmic signaling protein research).
Further questions:
[Troubleshooting Questions are selected from MCE customer consultation emails.]
Comparative analysis of RIPA and NP-40 lysis buffers:
- Differences in composition and lysis mechanism
- RIPA lysis buffer: uses a strong detergent system (including SDS, etc.), which can effectively lyse the cell membrane and nuclear membrane structure and destroy the intracellular biological membrane barrier.
- NP-40 lysis buffer: with non-ionic detergent as the core, it only gently lyses the cell membrane lipid bilayer, retaining the integrity of subcellular structures such as the nucleus.
- Differentiation of application scenarios
- RIPA lysis buffer: suitable for whole-cell protein extraction, especially for experiments that require simultaneous acquisition of cytoplasmic and nuclear proteins (such as nuclear transcription factor detection).
- NP-40 lysis buffer: focuses on cytoplasmic protein separation, suitable for scenarios that require retaining nuclear structure or avoiding nuclear protein contamination (such as cytoplasmic signaling protein research).
- The Protocol and precautions
The usage and precautions of RIPA and NP-40 lysis buffers can be obtained by entering the MCE product details page to obtain online technical documents (Manual Pdf):
- RIPA lysis buffer:
https://www.medchemexpress.com/inhibitor-kit/ripa-lysis-buffer-(strong).html
- NP-40 lysis buffer:
https://www.medchemexpress.com/inhibitor-kit/np-40-lysis-buffer.html
The answer to this question comes from MCE Technical Support.
How to contact us?
- Tel: 609-228-6898
- Fax: 609-228-5909
- E-mail: [email protected]
- Technical Support: [email protected]
- Address: 1 Deer Park Dr, Suite F, Monmouth Junction, NJ 08852, USA
- Badges
- Science topic
- Similar topics
- Psychology