Are there any fixation, staining, and separation protocols for cells cultured in Matrigel?
It would be best if it is for Matrigel of different thicknesses.
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If the matrix gel is coated with a thin layer, cells usually only grow on the surface because of its thinness, and can be directly fixed and stained; if the gel is thicker, cells may be embedded in the gel, and it is recommended to digest the matrix gel first.
Our (MedChemExpress, MCE) organoid separation solution (HY-K6015) can be used for such cell separation operations. Similarly, thin-layer coated matrix gel can also be used to achieve cell separation using this organoid separation solution.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
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