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Questions related from Heba Huessin
Hi. I activated qiime 2 2019, prepared a folder named '6 sample file' to try data import which has metadata, manifest, and 6 data sequences as in the picture. I received this error. maybe as...
10 October 2019 7,068 1 View
I am watching qiime importing metadata tutorial. I downloaded the barcode and sequence file using url and tried to open in qiime but they do not work as follow: (micro) a-PC:desktop hebahussein$...
10 October 2019 876 3 View
I prepared manifest file using all FASTQ sequences and now I need to prepare metadata file. Is it a must? Also, how to convert manifest file (csv) into .qza My facility gave me a yml file with...
10 October 2019 6,813 1 View
My lab told me that the sequences are demultiplexed but the manifest file I generated from illuminating basespace have only sample ID and direction as in picture. How to know if data need...
10 October 2019 3,309 1 View
If I used same power, type 1 and 2 errors from a past similar research, what is the point of recalculating sample size? Can I just use same sample size with reference to the research? On the other...
08 August 2018 5,734 2 View
is STROBE a reporting tool? so we need to make sure we reported every item of the checklist in our manuscript before submission? or we simply can mention that some items are not applicable to our...
08 August 2018 394 2 View
Or just one ethical approval.
08 August 2018 9,049 3 View
I got ethical approval for my research, but while submission, I noticed that editors comments need changing one word to the title into another word. Can I change it while I got ethical approval...
08 August 2018 4,064 9 View
any book or videos? i plan to have a training but need to understand the basics first. thanks.
06 June 2018 4,942 6 View
Hi. Any recommend youtube channel/website/ online course to understand the cycle of grant/budget planning/forming teams? Thanks
06 June 2018 2,646 0 View
any youtube channel/online courses/ website to help understand the cycle of grant application, budget planning, and team formation more than just simple google search? thanks
06 June 2018 3,629 1 View
in STROBE, they call for writing study design in title. I found it reluctant to write cross sectional study while the title already says prevalence of....
02 February 2018 6,278 7 View
We published a paper about a biomarker we extracted from patients sample, did PCR, found its expression. Now, we did bioinformatic using software which search the mechanisms of this marker. When...
12 December 2017 2,912 3 View
What are the most popular bone biomarker proteins for bone regeneration please? thanks in advance.
12 December 2017 3,036 3 View
Hi Can I test bone regeneration in vitro using cells other than bone stem cells? for example, using cancer cell line and testing bone biomarkers? thanks
12 December 2017 1,997 5 View
how can I draw a graph illustrating methodological steps of my experiment or a simplified pathway? I am searching for a more advanced program than powerpoint.
11 November 2017 9,039 2 View
..
02 February 2017 4,281 1 View
Would use Cochrane acrobat tool or there are easier tools.
02 February 2017 2,122 4 View
I know A refers to adult hemoglobin but don't know 1c.
12 December 2016 5,835 0 View
As I understand, locus is location of gene on chromosome. It is just location but the gene composed of nucleotides. But I read that genes as MHC has 6 loci. Also, I read multiple allele on same...
12 December 2016 8,668 0 View
Mainly flow cytometry, sequencing, elisa.
11 November 2016 9,481 1 View
Say we investigate prevelanve of diabetes mellitus in a dental setting and the previous studies use different sample sizes. Which sample size to use. As a rule of thumb, I know that 400 is enough...
11 November 2016 4,791 2 View
Attached diagrams from published paper and Internet and I first don't know if can cite without permission and second the form of citation.
11 November 2016 8,877 2 View
Since we don't have mean or SD as I don't have population, which test to use? Papers mention student test but don't know how. I have two bands for each marker. I quantified to get percentage...
10 October 2016 4,567 1 View
I followed the company dilution recommendation and it seems too dilutent. I have to normalize using many dilutions of primary antibodies to reach best results. What dilutions do you use for these...
09 September 2016 5,020 2 View
.
09 September 2016 1,546 1 View
The tips can be easily supplied as RNAse free but not other equipments.
09 September 2016 5,457 1 View
What concentration and duration .
09 September 2016 4,943 4 View
09 September 2016 5,635 0 View
Can't find any on YouTube or Jove.
09 September 2016 752 1 View
does keeping (not aspirating or decanting) medium and centrifuge , resuspend the pellet, and using these pellet together with scraped cells, give us important information about apoptotic cells may...
08 August 2016 2,941 1 View
Any video or PDF please to help read western results? .
08 August 2016 7,261 2 View
I already seeded and treated the cells in 6 well plate: 2 wells treated and four wells control (2 for positive control stain and 2 for negative control stain). How to collect and store these cells...
08 August 2016 2,401 2 View
Most steps need one or two hours of wait. Do you have tips to make use of wait time? Is there steps which can be done not in order during wait time.
07 July 2016 3,695 7 View
Is cell lysis protocol same for all proteins?.
07 July 2016 6,640 4 View
07 July 2016 1,009 2 View
my cells doubling time around one day and i cultured them four days ago and they did not double or increase in number. they did not die neither. may be the medium is not enough? .
07 July 2016 6,128 10 View
I suspect mycoplasma as cells stopped growing but I can't see the characteristic shape under microscope. Did this happen to anyone?.
07 July 2016 6,290 3 View
any tips to reduce the evaporation of ethanol while powder reconstitution? Will it continue evaporate after adding it to cancer line?.
07 July 2016 4,633 1 View
is it a new trend or journal guidelines? I read so many papers recently writing the methods after discussion.
07 July 2016 7,977 7 View
Please.
07 July 2016 9,430 0 View
and if i can treat it.
07 July 2016 6,080 0 View
Each one has disadvantage. DMSO kill the cells and ethanol evaporate so which one u use. K
07 July 2016 2,017 3 View
I need to transfer the flask to another lab ten minutes away to take pictures as my lab not have computer connected microscope and I failed to take pic using phone. The whole process may take one...
07 July 2016 4,725 0 View
i incubated two flask and second day I found a problem in co2 connection which dropped co2 level from 5 to 2%. Under microscope, one flask dead and the other healthy. Shall I trust the healthy...
06 June 2016 3,867 4 View
Is there a free software I can download on the computer to provide an online webinar?
06 June 2016 6,424 2 View
I have bubbles on my well plate and I think to remove using the hose and glass pipette same as we aspirate exhausted medium. I know I shall touch the wall of well but don't know why bubbles happened.
06 June 2016 8,214 2 View
I trypsin cells to do cell counting and seed in 96 well plate. Since I am a beginner, it takes so much time. Can I store the tube with cells in the incubator till I finish counting and then take...
06 June 2016 7,091 5 View
My lab microscope not connected to computer and I need to take pictures of the cells in different growing stages. Someone recommended the regular phone camera. Any tips for better details or any...
06 June 2016 8,941 4 View
do you count cells in two successive days and find the ratio or just observe by eye and estimate the doubling time? is doubling time fixed for the same cell culture? thanks.
06 June 2016 3,269 0 View
How to calculate the log.
06 June 2016 4,158 5 View
Vitamin d comes in brown bottle so no problem but once I open it and add the DMSO and aliqoute in small eoendorfs then store in -20 then back to hood to add to cells. How to protect it from light...
06 June 2016 9,056 0 View
When counting, I find cells close to each other like square A in the attached pic. Not over each other like square c. Is it considered clumb? I pipette several times but still cells close. Is it...
06 June 2016 1,465 4 View
Trypsin detach adherent cells from dish so we can transfer them. When to stop trypsin by adding medium? Is seeing moving round cells enough? Should cells move fast or slow? Do cells must move...
06 June 2016 1,429 3 View
I added the drug. I want to test cell growth. I will add PrestoBlue reagent to cells and read using micro reader on day one. I also want to test effect of drug on day two and three. Can I just...
06 June 2016 867 1 View
I dissolve my drug in dmso so I need to add dmso to the control group to exclude its effect. Is 1% dmso save to cell? I try to dissolve my drug in 1% DMSO but still need to find solution to dilute...
06 June 2016 8,414 2 View
Attached the pics I got. How to get these light circles out so I can see the cells through my iPhone? .
06 June 2016 6,805 0 View
I see some people pour the exhausted medium into a beaker which contains bleach placed inside the hood instead of suction. The idea seems good saving resources but was wondering if bleach can...
06 June 2016 7,299 0 View
I need to dissolve vitamin d in least possible volume of dmso not to kill the cells. But I need total volume of 1200 up to reach molar conc of vitamin d. Of this 1200 ul, what percent of dmso can...
06 June 2016 4,497 1 View
I use pubmed, Cochrane, goggle scholar when I search for papers but I wonder if most researchers use more databases especially when doing systematic reviews.
05 May 2016 4,257 3 View
I
05 May 2016 4,443 0 View
The goal of the experiment is to analyze cell cycle after adding a drug.nthanks
05 May 2016 8,074 6 View
How to calculate stock concentration
05 May 2016 8,516 2 View
If we have a drug in powder form, how can we calculate the amount needed for certain concentration when it converted to solution? For example 10 to power of -7 M concentration.
05 May 2016 468 0 View
Hi my lab protocol does not use trypan blue to count the cells. In my past lab, I used trypan so I could count the cells which did not take the blue dye. So how to differ dead and alive cells when...
05 May 2016 3,230 3 View
The the number of papers yield from PMC and free filters are different and out of curiosity, I would like to know the difference between the two features. Thanks
02 February 2016 6,578 0 View
What is the impact factor, if any, for cochrane ? Since cochrane is not a journal, I wonder if it has an impact factor.
02 February 2016 8,284 1 View
When to describe a confidence interval as narrow, and hence a large sample size or wide and hence a small sample size? Thanks
02 February 2016 4,744 6 View
Any suggested tips to plan for the reviewing process of case report paper in a journal? It is my first experience as a reviewer. Thanks
01 January 2016 9,531 3 View
Any recommended videos, articles, books to understand the data of DNA histogram produced by flow cytometry?
12 December 2015 6,467 2 View
Is there any scientific evidence that journals published on Thomsbon of more value than others?
12 December 2015 8,040 3 View
Any website /software/application to design awareness posters such as those directed to the patients?
12 December 2015 1,701 0 View
What does enrichment mean in flow cytometry?
12 December 2015 5,148 3 View
Why chicken erythrocytes were chosen in specific? And why human lymphocytes not other species?
12 December 2015 6,823 5 View
what is the scientific way to write a power point presentation as a reference in a scientific paper not to be published?
10 October 2015 8,401 3 View
I am reporting the results sections of different papers and I need to know the unit of measurement of PCR. Is it enough to report if the expression increased or decreased (binary)? If so, can I...
07 July 2015 9,757 4 View
Which of these two specialities ?
06 June 2015 8,864 2 View
And how to calculate sample size in those studies please?
06 June 2015 8,475 0 View
How to calculate sample, choose population in case control studies?
06 June 2015 7,906 0 View
Can you help me calculate these, please?
05 May 2015 5,673 5 View
Is there a minimum number or unacceptable low number of referrences used in the bibliography of a paper? Thank you
04 April 2015 5,184 3 View
Do most journals require 30% price index so that the percent of referrences within five years to all ref is 30% or it differs from journal to another?
04 April 2015 1,071 0 View
Sometimes there is a simple sentence in a paper and at the end it is cited by many references like for eg:23-30,33-35 . In this case, 11 references. When I parapharse this sentence and use it in...
04 April 2015 792 3 View
What is the difference between a CV and a biosketch ?
04 April 2015 5,682 0 View
I noticed some errors mendeley does and need to know if it is normal and we have to revise 1. Sometimes, it enters editor name instaed of author name 2. It always write the first initial only of...
04 April 2015 9,876 0 View
hi! Mendeley has only one field for title and one field for author. I have citation which is chapter in book written by different author. How to cite it in mendeley?
04 April 2015 3,038 8 View
I found a citation in a paper I am reading from CDC about frequency of a disease. I am not sure it is cited correctly because it does not have date . It was as follow: National Hospital Discharge...
04 April 2015 1,175 1 View
Is there a general rule where researcher consider while writing the paper not to include old references ? How many years ago considered old? What the percentage of these old references in relation...
04 April 2015 6,625 7 View
I know there are different methods to calculate sample size, but I need this one, please!
03 March 2015 7,130 5 View
I need to deliver a training regarding scientific writing and other skills and I need a tool where I can communicate with my team and at the same time has illustrative capacities like board . Any...
03 March 2015 876 2 View
I need those websites to paraphrase my own paraphrase for the main paper.
02 February 2015 3,738 1 View
I hear a lot that ONE study can be published in many papers. May be they choose different outcome, or duration. How this not considered plaigrism? On journals guidelines, it is mentioned that the...
02 February 2015 5,378 3 View
Can you please share your experience in publishing oral medicine systematic reviews? Are there journals accept papers with little or no fee?
02 February 2015 3,071 3 View
Thank you in advance
02 February 2015 1,153 7 View
I tried to paste pieces of articles to check the accuracy of both : paperrater.com and plagiarisma.net but neither could not discover plagiarism. Some suggested turnitin, but as far as I tried,...
02 February 2015 6,472 2 View
I have group of names of authors. But to limit the search, I need to search by speciality and year of publication as well. In Pubmed, there is option to search by year of publication but it needs...
02 February 2015 6,397 4 View
On writing SR, do you exclude non-english publications in case of lack of resources for translation? Or is there another alterantive! Thank you!
02 February 2015 4,464 0 View
Can you please suggest, based on your experience, dental non predatory journals accept publications without fee?
02 February 2015 3,651 2 View
How should I use ( I mean the guidelines ) and how should I cite ( I mean how to write the source) a picture or diagram from any website without violating the copy rights? Thank you.
02 February 2015 2,956 5 View
I tried proquest, but it is not free. Is there any free alternative?
02 February 2015 3,999 2 View
Do you use revMan to write your review? Or you just use it for generating tables, graphs, references then copy and paste in word file?
02 February 2015 9,121 3 View
I am just curious. Do you follow potentional journal guidelines while writing or fo you finish writing then adapt the journal format? And if the paper rejected by a journal, is there a way to...
02 February 2015 6,438 1 View
Which approach do you take? After writing my article, I chosed the journal which published a relevant article from my references; however, the journal uses a very different format. Do you choose...
02 February 2015 5,934 22 View
And what is the importance that a journal be on ISI and how to check?
02 February 2015 184 2 View
I believe choosing the topics a the most difficult step. What is the recommend steps of thinking and search? Do we choose topic we are intersted in or most trendy? Can we choose a topic which...
02 February 2015 7,778 9 View
Is there any quidelines or steps/ procedure for writing a paper summarizing guidelines about treatment of certain disease? Thank you in advance!
02 February 2015 4,478 0 View
I read a myriad of opinions around R. Some say it is more important than SPSS, while others say it is obsolete.
02 February 2015 1,651 9 View
I read chapter 8 many times in cochrane handbook, but there are certain domains I can't fahom in risk of bias tool 1. What diff between blinding and concealment? Blinding can easily mentioned in...
02 February 2015 2,717 3 View
I find difficulty applying all items of PRISMA while writing SR. which review guidelines of ttt. It does not include any RCTs, so I can't apply Nany items such as risk of bias and its results. The...
02 February 2015 902 6 View
While searching for the primary studies which are RCT as inclusion criteria for SR, there are many studies based on the theoretical basis or explain a certain mechanism. I need these later studies...
02 February 2015 9,019 3 View
I am confused about the function of PRISMA. is it a guide for SR author to follow, or a method to evaluate the studies which will be included in his SR? Also, I find PRISMA very general method and...
02 February 2015 9,240 7 View
After reading the cochrane handbook and some articles I am still confused between those two domains.
02 February 2015 6,724 8 View
Some advocate for effect size, and others for confidence interval. Which is more reliable?
01 January 2015 5,907 6 View
I want to publish a paper without a fee. I was referred to i DOJA. apart from speciality, what other criteria shall I look for when choosing a journal? I read that IF is not the most reliable...
01 January 2015 5,127 2 View
I am reading a statistics book and there is example that odds ratio8 and CI 1-15 . Here the odds ration is not 1 but CI includes 1 . So, do we consider it statistically significant or not?
01 January 2015 3,567 4 View
Can a paper has two corresponding authors if they are doing the same amount of work?
01 January 2015 1,147 3 View
hello! How can I encounter and contact publishers who recruit members for their research teams using ResearchGate?
01 January 2015 701 0 View
Is there any journal which accept publishing without publishing fee? ( the publication fee itself not the open acces fee) thank you in advance
01 January 2015 8,566 3 View
Is it true that most editors prefer statistically significant result? If so, how do you deal with this fact? We do research as honest as possible whatever the result is. Of course, some...
01 January 2015 3,617 5 View
i, mistakenly, mixed two flasks of t-25 into t-75 flask. both same cell line but got from different vials. one was slower than the other. how this will affect my results?
01 January 1970 6,079 4 View
What percent of old papers is allowed.
01 January 1970 7,798 5 View
for example. how to cite info taken from this URL Source: https://www.quora.com/How-are-microRNAs-named
01 January 1970 1,316 3 View
If I adapted my questionnaire from a previous published paper (used the same questions with minor changes), is it enough to ensure validity? what the practical steps to ensure validity of...
01 January 1970 760 1 View
01 January 1970 2,871 0 View
some journals ask for a running title. what does this mean and how to write it.
01 January 1970 7,725 18 View
Hi. 1. Why do we use internal (negative ) control as actin? 2. Why we don't attach the detector enzyme directly to primary antibody?how secondary ab amplify the signal? 3. Why we need to separate...
01 January 1970 9,147 4 View
I seed cells in dish then next day apply treatment then after three days collect protein. How many estimate cells to seed.
01 January 1970 7,553 3 View
I am doing western for these proteins and need to know expression level to determine how much cells to grow and how much lysis buffer.
01 January 1970 1,889 5 View
I treat cells then compare protein expression compared to untreated. If I test five protein, do I need to seed in five well of six well plate or just one.
01 January 1970 3,292 5 View
I am testing effect of drug on expression of five proteins. I guess I shall use five wells. Each for one protein and the sixth well for control with no drug. I don't know how many cells to seed...
01 January 1970 9,183 2 View
Any alternative device or simple tool to these devices to increase protein concentration for western blot? Taking into account, the maximum signal used and minimum lysis buffer used.
01 January 1970 1,356 7 View
I don't know the optimal antibody concentration so I may not reach good result and need to try another concentration (higher) using same membrane the next day so need to know how to store the...
01 January 1970 4,751 2 View
1. Do you keep the ice box in the hood or outside while washing with PBS and adding lysis buffer? 2. How much time you leave lysis buffer before scraping? 3. How much time you centrifuge? i...
01 January 1970 4,167 4 View
On imaging, there was very faint lines. I am not sure they considered bands. I used beta actin and p-53. Is it possible that cell lysis not worked at all? I did BCA and got reading for the...
01 January 1970 9,555 4 View
Other than that hand made gel is cheaper, is there any other advantage ? . Or better to use ready? .
01 January 1970 2,225 3 View
01 January 1970 1,818 0 View
i add 7 ul of biorad color standard. Don't know how much lamalli or beta mercapoethanol to add to it.
01 January 1970 8,314 5 View
I add lamalli buffer to sample ratio 1:1 then beta mercapo as 10% of total volume (50ug) but don't know how to add protein marker (standard precision protein).
01 January 1970 6,116 6 View
What the function of using two wells with the loading buffer only? Thanks.
01 January 1970 9,276 4 View
The picture shows guide for choosing gel % but there is overlap between protein sizes. Also don't know which to choose if have proteins of different sizes. Tahnks
01 January 1970 9,715 6 View
Attached is pic for ab. When I cropped, the resolution became less.
01 January 1970 6,476 1 View
i centrifuged at 14000 rpm 20 min but not pellet formed.
01 January 1970 1,191 6 View
I don't like the machine pictures as they are so faint even with five minute exposure so I need use x ray.
01 January 1970 3,383 1 View
Attached a pic. I don't know why this happened. BTW, this is done on stripped blot. But first time the blot was negative .
01 January 1970 7,138 2 View
I need to repeat unfortunately for many reasons: either conc of antibody or expired ab or human error. I may need to use same piece of membrane for two more trials. Is it possible? .
01 January 1970 1,804 4 View
My proteins appeared but actin not. May be I forgot to add the secondary antibody to the wash buffer or primary ab actin was expired. So I need to repeat the all antibodies with new gel run? I...
01 January 1970 6,542 3 View
I quantified the bands using studio image lite . I have treated group and untreated group. Say the treated is 10% of the untreated. How to calculate p value?
01 January 1970 2,961 4 View
01 January 1970 8,496 0 View
01 January 1970 3,618 1 View
01 January 1970 4,736 0 View
Is it reproducible and accurate to do primers?.
01 January 1970 2,077 3 View
this is my first time to teypsinize this cell line. I added 0.7 ml trypsin to 25 flask and observed from 2-8 minutes but most of cells did not detach. The trypsin works good as I used with other...
01 January 1970 4,774 5 View
I consider buying cell line but it mentioned it contaminated with another cell line. Is it better not to buy it? Also, I have cell line in lab but it discontinued (not sold by AtCC anymore, is...
01 January 1970 8,621 0 View
prestoblue not work for one of my cell lines. i use absorbance 570 and 600 but need to perform spectrum scan on multi plate reader but dont know how.
01 January 1970 3,869 1 View
What is the origin of cell line? For example, I have cell lines from JHU cancer cells. From metastasis oral scc and laryngeal scc. They share similar characters as morphology and response to...
01 January 1970 4,597 4 View
What the regulations to carry cell line on dry ice?.
01 January 1970 4,839 1 View
01 January 1970 9,876 1 View
I know that the prepared medium shoul be used within three weeks only of prepration then discarded but a college told me that the FBS is even more sensitive and should not last more than two...
01 January 1970 5,572 3 View
I feel frustrated doing silly mistakes as forgetting to loosen the cab of unfiltered flask for example . Is it normal to continue doing these mistakes.
01 January 1970 2,044 6 View
these photos taken by phone for cells at day 4 and day 7 culture. shall i transfer that in picture 2 to bigger t-75 flask or not yet?
01 January 1970 7,605 12 View
In first picture (low magnification), can we consider the two black cells on top corners apoptotic? In second picture is high magnification of one of the cells and also appear dark.
01 January 1970 2,855 5 View
I did PrestoBlue cell growth and proliferation assay and the numbers of drug group is smaller than the medium so the treated group-medium is negative. Does this mean all cells died ? But under...
01 January 1970 6,344 5 View
all my cell lines detached within five minute trypsin except one. i dont know which is better for cells and less harsh: to increase amount of trypsin from .7 to 1 ml for t-25 flask or to increase...
01 January 1970 4,375 8 View
01 January 1970 7,377 2 View
01 January 1970 8,375 0 View
some journals ask authors to suggest reviewers. I wonder how can it be unbiased reviewing in case the reviewer knows the author.
01 January 1970 4,057 5 View
I am frustrated. I need to know how everyone is learning, do you watch someone doing the experiment? Does someone observe and correct you? How physicians master research especially bench research ?.
01 January 1970 4,148 3 View
if i copy and paste the name as it in paper (first last) or even last, first, the name inside the word documents just first letters for first and last names. what the correct name to enter...
01 January 1970 6,990 3 View
I am new to bioinformatics and newer to QIIME. I have an excel file with sequenced/ demultiplexed data and I need to use QIIME to analyze the data bt I need to know what it actually does. does it...
01 January 1970 2,106 4 View
how
01 January 1970 7,533 0 View
...
01 January 1970 10,017 2 View
especially websites and youtube please. TIA
01 January 1970 3,374 5 View
01 January 1970 9,330 0 View
01 January 1970 2,986 2 View
I have limited time so I may search two proteins to test the expression. Can I test only two and still my data be valuable .
01 January 1970 9,619 1 View
Do I need to aliqoute protein? Also, by mistake while doing BCA assay, I boiled protein above 100.
01 January 1970 7,111 2 View
And what will be the scientific benefits for me to register.
01 January 1970 7,705 0 View
how to know from mean and SD if data parametric or non parametric? And how to know for nominal data (yes, no)? .
01 January 1970 7,103 2 View
After centrifuge and discard supernatant, I mix pellet of cells with 10 ml medium and count. I take average and multiply by 10000 to get number of cells in ml so I know how many cells to seed in...
01 January 1970 3,142 2 View
We all now know that vit d is promising cytotoxic drug but is it cytotoxic also to normal cells (mainly oral cells).
01 January 1970 309 1 View
And how much not having positive control would affect the result? .
01 January 1970 5,045 4 View
I have the big brown bottle of RIPA and it takes ages to thaw. How to thaw safely? I know I have to aliquote but concerned to put in eppendorf as it light sensitive.
01 January 1970 7,820 5 View
People always talk about scopus and Thompson as standards. What do you think about Sci Forschen in particular? I don't believe that high impact is the only criteria or even the most important for...
01 January 1970 5,690 1 View
Would it add a value to the finished review? As for example it will ascertain no reporting bias.
01 January 1970 7,730 1 View
hi attached a paper to test miRNA expression. can I pick one assay to study miRNA expression (Reverse Transcriptase PCR) or i have to do the others as FISH, transfection? i am doing miRNA among...
01 January 1970 5,544 4 View
Attached picture of nanodrop result. I used qiagen miRNeasy mini kit to extract RNA . I will use it further for miRNA profiling.
01 January 1970 4,482 6 View
Nanostring has many assays to test miRNA profile and I don't know which to order.
01 January 1970 9,303 1 View
Hi attached picture of miRNA sequence in a paper. Then a picture of it from miRBASE. The seq in the paper easy to find and understand but where it is exactly on the miRBASE ? Thanks.
01 January 1970 4,679 3 View
What miRNA profile refers to.
01 January 1970 766 0 View
some miRNAs decrease proliferation of cancer cells. in this case, does the use of thearpuetic agnets which upregulate these miRNA can help treat cancer? and what is the difference between...
01 January 1970 6,281 3 View
Do you prefer taqman or syber green PCR kits? .
01 January 1970 942 1 View
01 January 1970 1,632 0 View
01 January 1970 3,615 0 View
01 January 1970 8,681 3 View
01 January 1970 6,962 1 View
01 January 1970 3,672 0 View
What does the colors indicate in any heat map for miRNA profile. How to understand the Heatmap from just looking. Attached a picture from a paper.
01 January 1970 8,367 0 View
I have 800 miRNA but about 20 up and 20 down regulated more than 2 fold change between the control and treatment group. I need to perform heat map but don't know which columns to import and whites...
01 January 1970 281 2 View
Does copying from thesis to a paper considered self plagiarism ? .
01 January 1970 2,232 14 View
i use johns hopkins university head and neck cancer cell line. my lab had it as a gift so i dont know its characters: IC-50, doubling time, passage numbers, etc.
01 January 1970 6,181 0 View
I pellet my cells at 1000 rpm and using RNA isolation which require 12,000 xg. How to convert this 12,000 xg to rpm .
01 January 1970 11,741 26 View
this seems trivial question but label disappear on boiling and samples may mix.
01 January 1970 9,060 5 View
Most paper list ALL materials and their companies at the first part of methodology but I find it boring . I prefer to mention what I used when I am telling what I did. Eg: in western section, I...
01 January 1970 3,562 0 View
I will use 70% alcohol for 20 minutes to induce apoptosis but I need reference to write .
01 January 1970 3,551 1 View
the Cochrane seven risk of bias domains: random sequence generation, allocation concealment, etc are repeated in the review and may result in high similarity index in the plagiarism software. is...
01 January 1970 3,087 0 View
I test the drug at 6, 12, 24 hours but find difficulty seed the cells at same day as 12 hours group will require me stay at lab 12 hrs or come very early next day. Can I do them as separate...
01 January 1970 7,585 4 View
I am a dentist and a researcher. I grow cells, extract DNA and RNA, collect samples from patients, design studies. my following project involves sequencing (NGS) and I wonder is it necessary to...
01 January 1970 4,602 22 View
Any?.
01 January 1970 4,256 0 View
I don't know what causes this black area in my cells.
01 January 1970 5,250 3 View
I don't know how to get a list of these conferences. I want to apply for poster.
01 January 1970 8,878 0 View
As I understand, cronbach alpha is calculated AFTER we get the responses of participants and not before so how we calculate it?
01 January 1970 9,061 4 View
I don't know if I shall use the dilution recommended by the company or the higher concentration, the more possibility protein will be detected?.
01 January 1970 5,487 3 View
I use biorad marker . Most antibodies m wt around 40/50. I plan cut horizontally to use 3 antibodies per membrane but the m Wt so similar. Can't cut vertically as I have 7 samples. Attached picture .
01 January 1970 225 3 View
Is first picture mycoplasma? Or just ink image from writing on flask? i believe the second picture (has regular circles) is from the microscope lens. But don't know how to get it out of the field.
01 January 1970 3,880 2 View
01 January 1970 2,219 0 View
01 January 1970 1,982 0 View
I read two opposite opinions: one says membrane should kept wet all time after transfer and they keep on adding wash buffer before stain and another opinion says that it should be dryed.
01 January 1970 3,542 0 View
Ethanol used to induce apoptosis so why it does not kill the cells when used for fixation?.
01 January 1970 5,598 1 View
01 January 1970 2,293 5 View
For virtual one, I plan to resent just one of 4 methodology to be used in actual one but the title is same.
01 January 1970 9,551 0 View
which should be first? Paper submission to journal. Acceptance, poster submission to conference, poster presentation or it does not matter?.
01 January 1970 9,829 2 View
What is the difference in action and theory.
01 January 1970 563 3 View
01 January 1970 4,337 0 View
the tech told me he just knows how to run the machine but not to add the annexin or PI.
01 January 1970 4,321 0 View
Attached a scheme for the groups (flasks): one flask positive control, one group treated with the drug, one group (flask) treated with medium (live or negative control). I need to use BD apoptosis...
01 January 1970 2,253 4 View
01 January 1970 4,781 3 View
01 January 1970 8,510 1 View
I created risk of bias tables in RevMan in order to use them to create risk of bias figure. Now, I need to attach these tables and figures in my word document to be sent to the journal. How to...
01 January 1970 6,228 6 View
albumin concentration 2mg/ml and volume of vial 1 ml. I need five concentration to get curve. I don't know how much volume or concentration in each vial. Also I don't know how much buffer to add...
01 January 1970 9,816 1 View
01 January 1970 1,187 0 View
i know it is totally by experience but what the guidance? especially some areas may be more condense as border of flask and sometimes cells round or branching. sometimes cells overlapping.
01 January 1970 9,644 2 View
Beta actin I used was expired so did not show but other antibodies like cyclin were good. Can I strip and re add ? For VDR, conc may was low so I need to inc conc.
01 January 1970 799 1 View
And any alternative to triton x? I have PI at lab but not triton.
01 January 1970 2,666 0 View
Is 100 mm dish means diameter is 100 mm? If so, surface area of circle 3.14 *50^2 not equal 55 cm^2 I need to know as I need to know how much RIPA lysis buffer to add to well of six well plate....
01 January 1970 4,179 2 View
Is it possible that just microscope view of seeded cells in 96 or 6 well plate can give proof (for ourselves not for paper) that the cytotoxic drug caused less cell numbers? It it can't be done as...
01 January 1970 1,102 2 View
01 January 1970 6,367 0 View
