Do you allow PVDF membrane to dry after membrane transfer or drying hurts the membrane?

01 January 1970 0 4K Report

I read two opposite opinions: one says membrane should kept wet all time after transfer and they keep on adding wash buffer before stain and another opinion says that it should be dryed.

Badges
Science method

More Heba Huessin's questions See All

Importing files into qiime 2 not working. any clue?

I am watching qiime importing metadata tutorial. I downloaded the barcode and sequence file using url and tried to open in qiime but they do not work as follow: (micro) a-PC:desktop hebahussein$...

09 October 2019 942 3 View

How to know if my sequence data demultiplexed?

My lab told me that the sequences are demultiplexed but the manifest file I generated from illuminating basespace have only sample ID and direction as in picture. How to know if data need...

09 October 2019 3,374 1 View

How to convert files into .qza for qiime analysis? and how to prepare/find metadata/ mapping file?

I prepared manifest file using all FASTQ sequences and now I need to prepare metadata file. Is it a must? Also, how to convert manifest file (csv) into .qza My facility gave me a yml file with...

09 October 2019 6,892 1 View

Importing paired FASTQ file into qiime had errors. any help?

Hi. I activated qiime 2 2019, prepared a folder named '6 sample file' to try data import which has metadata, manifest, and 6 data sequences as in the picture. I received this error. maybe as...

09 October 2019 7,127 1 View

Can we change one word in the manuscript title after ethical committee (IRB) approval?

I got ethical approval for my research, but while submission, I noticed that editors comments need changing one word to the title into another word. Can I change it while I got ethical approval...

07 August 2018 4,108 9 View

Do we must write/fill ALL items of STROBE checklist in our manuscript?

is STROBE a reporting tool? so we need to make sure we reported every item of the checklist in our manuscript before submission? or we simply can mention that some items are not applicable to our...

07 August 2018 477 2 View

Do we really need to calculate sample size?

If I used same power, type 1 and 2 errors from a past similar research, what is the point of recalculating sample size? Can I just use same sample size with reference to the research? On the other...

07 August 2018 5,796 2 View

In case of multi-center studies, do we need an ethical approval from each center (university)?

Or just one ethical approval.

07 August 2018 9,088 3 View

Any recommended sources for grant application writing please?

Hi. Any recommend youtube channel/website/ online course to understand the cycle of grant/budget planning/forming teams? Thanks

05 June 2018 2,698 0 View

Any recommended sources to understand grant application writing?

any youtube channel/online courses/ website to help understand the cycle of grant application, budget planning, and team formation more than just simple google search? thanks

05 June 2018 3,684 1 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Is the black sediment consider as sand? If not, how can I filter it out?

Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...

30 July 2024 557 2 View