Why chicken erythrocytes were chosen in specific? And why human lymphocytes not other species?
HI
Chicken erythrocyte nuclei used as internal controls in order to calculate the ploidy because CEN have less total DNA per cell than human cells (35%).
Hope this helps
HI
Chicken erythrocyte nuclei used as internal controls in order to calculate the ploidy because CEN have less total DNA per cell than human cells (35%).
Hope this helps
Thanks Sreedhar. Does this mean the DNA simpler so reading the histogram easier?
HI
Relative positions (channel numbers) of the CEN peaks can therefore be used to determine the relative DNA index of unknown samples. CEN can be used if a ploidy check is deemed necessary while at the same time cell cycle data is required.
There is considerable debate as to whether CEN controls are the most appropriate internal standards. A human blood preparation may be more relevant, providing that the cell lines in question are not true diploid in nature (in which case the two peaks will overlap owing to their identical relative DNA contents
Hope this helps
Hi
I think in answer Erin, use what available in your lab.
The erythrocytes had dye binding characteristics similar to those of human
lymphocytes and a 20% lower fluorescence, thus being well suited as an internal
standard, as was demonstrated in tumor ploidy analyses performed with varied tissue concentration and appeared to be suitable and easy
to store frozen.
For accuracy it is better to use cells other than human like chicken.
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