Vitamin d comes in brown bottle so no problem but once I open it and add the DMSO and aliqoute in small eoendorfs then store in -20 then back to hood to add to cells. How to protect it from light during all these steps?
I prepared manifest file using all FASTQ sequences and now I need to prepare metadata file. Is it a must? Also, how to convert manifest file (csv) into .qza My facility gave me a yml file with...
09 October 2019 6,446 1 View
My lab told me that the sequences are demultiplexed but the manifest file I generated from illuminating basespace have only sample ID and direction as in picture. How to know if data need...
09 October 2019 3,106 1 View
I am watching qiime importing metadata tutorial. I downloaded the barcode and sequence file using url and tried to open in qiime but they do not work as follow: (micro) a-PC:desktop hebahussein$...
09 October 2019 683 3 View
Hi. I activated qiime 2 2019, prepared a folder named '6 sample file' to try data import which has metadata, manifest, and 6 data sequences as in the picture. I received this error. maybe as...
09 October 2019 6,872 1 View
I got ethical approval for my research, but while submission, I noticed that editors comments need changing one word to the title into another word. Can I change it while I got ethical approval...
07 August 2018 3,891 9 View
is STROBE a reporting tool? so we need to make sure we reported every item of the checklist in our manuscript before submission? or we simply can mention that some items are not applicable to our...
07 August 2018 236 2 View
If I used same power, type 1 and 2 errors from a past similar research, what is the point of recalculating sample size? Can I just use same sample size with reference to the research? On the other...
07 August 2018 5,572 2 View
Or just one ethical approval.
07 August 2018 8,898 3 View
Hi. Any recommend youtube channel/website/ online course to understand the cycle of grant/budget planning/forming teams? Thanks
05 June 2018 2,495 0 View
any youtube channel/online courses/ website to help understand the cycle of grant application, budget planning, and team formation more than just simple google search? thanks
05 June 2018 3,468 1 View
Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...
03 March 2021 6,223 5 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
28 February 2021 7,738 3 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
Looking for method of extracting and analyzing vitamin D in foods by HPLC
24 February 2021 7,524 7 View
We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...
23 February 2021 7,194 4 View
As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...
23 February 2021 3,175 3 View
I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...
22 February 2021 10,099 5 View