In western blotting, if results were negative, can I strip membrane pieces and add different concentration and/or company of same antibody?

01 January 1970 1 822 Report

Beta actin I used was expired so did not show but other antibodies like cyclin were good. Can I strip and re add ? For VDR, conc may was low so I need to inc conc.

Akash Sabarwal

Ofcourse You can do that Heba

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