Which websites you use to match miRNA primers and look for/confirm sequences ?

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Importing files into qiime 2 not working. any clue?

I am watching qiime importing metadata tutorial. I downloaded the barcode and sequence file using url and tried to open in qiime but they do not work as follow: (micro) a-PC:desktop hebahussein$...

09 October 2019 942 3 View

How to know if my sequence data demultiplexed?

My lab told me that the sequences are demultiplexed but the manifest file I generated from illuminating basespace have only sample ID and direction as in picture. How to know if data need...

09 October 2019 3,374 1 View

How to convert files into .qza for qiime analysis? and how to prepare/find metadata/ mapping file?

I prepared manifest file using all FASTQ sequences and now I need to prepare metadata file. Is it a must? Also, how to convert manifest file (csv) into .qza My facility gave me a yml file with...

09 October 2019 6,892 1 View

Importing paired FASTQ file into qiime had errors. any help?

Hi. I activated qiime 2 2019, prepared a folder named '6 sample file' to try data import which has metadata, manifest, and 6 data sequences as in the picture. I received this error. maybe as...

09 October 2019 7,127 1 View

Can we change one word in the manuscript title after ethical committee (IRB) approval?

I got ethical approval for my research, but while submission, I noticed that editors comments need changing one word to the title into another word. Can I change it while I got ethical approval...

07 August 2018 4,108 9 View

Do we must write/fill ALL items of STROBE checklist in our manuscript?

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07 August 2018 477 2 View

Do we really need to calculate sample size?

If I used same power, type 1 and 2 errors from a past similar research, what is the point of recalculating sample size? Can I just use same sample size with reference to the research? On the other...

07 August 2018 5,796 2 View

In case of multi-center studies, do we need an ethical approval from each center (university)?

Or just one ethical approval.

07 August 2018 9,088 3 View

Any recommended sources for grant application writing please?

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05 June 2018 2,698 0 View

Any recommended sources to understand grant application writing?

any youtube channel/online courses/ website to help understand the cycle of grant application, budget planning, and team formation more than just simple google search? thanks

05 June 2018 3,684 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

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Strugglling with m6A dot blot any suugesstion ?

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10 August 2024 8,539 5 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

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08 August 2024 6,134 12 View

How to confirm the site-directed mutagenesis result without performing NGS?

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RNA Extraction Using Hot Borate Method No Longer Working?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

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Ravichandran Panchanathan

hi, you can visit thermo fisher company and look Oligos, Primers, Probes & Nucleotides section. if you want make custume primer sequence they will provided.

www.thermofisher.com

Asif Nakhuda

Try Ensembl

Alfred Simkin

I would agree that thermofisher is useful for primers (although it's been years since I've needed to design a primer). To look for/confirm miRNA sequences, you should definitely use mirbase. They have a downloads section that allows you to download all miRNA sequences across all species that mirbase has on file.