Even if the status of p53 is known in the cell line, one may require to optimize the concentration of hydrogen peroxide for a given time. Additionally, culture conditions (medium and growth factors) may influence the outcome.
Mr. Divaker Choubey the same rights, but we must take into account:
- Status of p53 is associated with lymphocyte apoptosis mechanism.( p53 receptor and caspase-dependent apoptosis pathway in the implementation of lymphocytes induced by hydrogen peroxide).
One of the sources of reactive oxygen species in cells of the hydrogen peroxide to induce cell apoptosis and necrosis of the nervous tissue.
Apoptotic mechanisms include the action of hydrogen peroxide, from signaling pathways involving kinase PI3K, p38MAPK, JNK and ERK. Oxidative stress causes an increase in the synthesis of heat shock proteins, HSP70 and including exogenous may reduce the formation of reactive oxygen species in the cells (four cell lines, including JHU-022, JHU-028, JHU-029, SCC25 tested)
The experiment showed that it reduces the toxic effect of HSP70 as hydrogen peroxide and amyloid.
Important role in the mechanism by which HSP70 protects cells from apoptosis induced JHU peroxide, PI3K protein kinase and belongs to JNK.
The simple answer to your question is not
Use different doses of Hydrogen peroxide starting from 100 micromolar to 1 mM, In my opinion you can use 100-500 and 1000 micromolar concentration, see which one gives best result. Can also do a time kinetics with only one dose 3,6,12,24 h.
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