How to convert files into .qza for qiime analysis? and how to prepare/find metadata/ mapping file?

I prepared manifest file using all FASTQ sequences and now I need to prepare metadata file. Is it a must? Also, how to convert manifest file (csv) into .qza My facility gave me a yml file with...

09 October 2019 6,446 1 View

How to know if my sequence data demultiplexed?

My lab told me that the sequences are demultiplexed but the manifest file I generated from illuminating basespace have only sample ID and direction as in picture. How to know if data need...

09 October 2019 3,106 1 View

Importing files into qiime 2 not working. any clue?

I am watching qiime importing metadata tutorial. I downloaded the barcode and sequence file using url and tried to open in qiime but they do not work as follow: (micro) a-PC:desktop hebahussein$...

09 October 2019 683 3 View

Importing paired FASTQ file into qiime had errors. any help?

Hi. I activated qiime 2 2019, prepared a folder named '6 sample file' to try data import which has metadata, manifest, and 6 data sequences as in the picture. I received this error. maybe as...

09 October 2019 6,872 1 View

Can we change one word in the manuscript title after ethical committee (IRB) approval?

I got ethical approval for my research, but while submission, I noticed that editors comments need changing one word to the title into another word. Can I change it while I got ethical approval...

07 August 2018 3,891 9 View

Do we must write/fill ALL items of STROBE checklist in our manuscript?

is STROBE a reporting tool? so we need to make sure we reported every item of the checklist in our manuscript before submission? or we simply can mention that some items are not applicable to our...

07 August 2018 236 2 View

Do we really need to calculate sample size?

If I used same power, type 1 and 2 errors from a past similar research, what is the point of recalculating sample size? Can I just use same sample size with reference to the research? On the other...

07 August 2018 5,572 2 View

In case of multi-center studies, do we need an ethical approval from each center (university)?

Or just one ethical approval.

07 August 2018 8,898 3 View

Any recommended sources for grant application writing please?

Hi. Any recommend youtube channel/website/ online course to understand the cycle of grant/budget planning/forming teams? Thanks

05 June 2018 2,495 0 View

Any recommended sources to understand grant application writing?

any youtube channel/online courses/ website to help understand the cycle of grant application, budget planning, and team formation more than just simple google search? thanks

05 June 2018 3,468 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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02 March 2021 932 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

Data Analysis K-S and S-W tests?

Hello, I have conducted analysis where the K-W and S-W have come out as p

25 February 2021 9,648 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Statistics: include groups of respondents made by one person for analysis?

Good morning, I could not find literature about this so I am asking. I am organizing data for a questionnaire (N=100) that foresees some personal questions like level of education. For the aim...

25 February 2021 2,090 4 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View