When fixing cells with ethanol for PI staining for flow, why ethanol does not kill the cell?

01 January 1970 1 6K Report

Ethanol used to induce apoptosis so why it does not kill the cells when used for fixation?.

Dear Heba,

It is just a difference of concentration. Fixation needs high concentrations to dehydrate the preparations and to kill the cells whereas low concentrations induce apoptosis of some cells.

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