Hi. I activated qiime 2 2019, prepared a folder named '6 sample file' to try data import which has metadata, manifest, and 6 data sequences as in the picture. I received this error. maybe as manifest and metadata are not qza (I read the tutorial but could not find how to convert into qza. Thanks a lot
(1/1?) no such option: -q
(qiime2-2019.7) a-PC:~ hebahussein$
(qiime2-2019.7) a-PC:~ hebahussein$ qiime tools import \
> --type 'SampleData[PairedEndSequencesWithQuality]' \
> --input-path 6 sample import \
> --output-path paired-end-demux.qza \
> --input-format PairedEndFastqManifestPhred64V2
Hi Heba Huessin ,
You don't have to convert the files into qza, QIIME can identify fastq files.
The image you provide shows that your fastq sequences are in zipped folder. unzip the file before proceeding further.
One more thing, you can not use metadata in yml format for QIIME analysis. You need to create a valid "mapping file" in QIIME's preferred txt format.
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