Is there any advantage of tagman kits over syber green kits for miRNA?

01 January 1970 1 1K Report

Do you prefer taqman or syber green PCR kits? .

I've only used SYbR green kits and they work fine. But they do have a limitation, in that SYBR just non-specifically binds to any double stranded molecule. So even non-specific amplicons or primer dimers (if significant in amounts) can interfere in the readouts you get. Taqman avoids this by using primer-probe conjugates, so only your specific amplicons contribute to the signal you get.

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