After centrifuge and discard supernatant, I mix pellet of cells with 10 ml medium and count. I take average and multiply by 10000 to get number of cells in ml so I know how many cells to seed in well plate . But can't figure out how to calculate how much cells were grown on full flask? For example, if average number after counting was 25 cell. How many cells my full con fluent t-75 flask has?.
Dear Heba
1. The average number of cells per ml as you say is 25 x 10(^4) based on counting = 2.5 x 10(65)
2. As you say the total number of cells in the flask was pelleted and re suspended in 10mls of medium
3. Thus the total number of cells in your pellet = total number of cells recovered from flask = 2.5 x 10(^5) x 10 = 2.5 x 10(^6) = 2.5 million
Dear Heba
1. The average number of cells per ml as you say is 25 x 10(^4) based on counting = 2.5 x 10(65)
2. As you say the total number of cells in the flask was pelleted and re suspended in 10mls of medium
3. Thus the total number of cells in your pellet = total number of cells recovered from flask = 2.5 x 10(^5) x 10 = 2.5 x 10(^6) = 2.5 million
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