Hi Can I test bone regeneration in vitro using cells other than bone stem cells? for example, using cancer cell line and testing bone biomarkers? thanks
Dental follicle stem cells / Dental Pulp Stem Cells have osteogenesis capability which you may use to test for bone regeneration.
Hi Heba,
Yes, definitely you can use other cell types. If you have the chance of working with mouse cells, I can suggest the preosteoblast MC3T3-E1 cell line. These cells can grow in α-MEM with 2mM Glutamine, 10% FBS, and 1% antibiotic solution (growth medium). You can differentiate them into osteoblasts if you culture them in differentiation medium (we were using the growth medium supplemented with 10 mM b-glycerol phosphate and 50 ug/mL ascorbic acid). Then, you can perform PCR, measure ALP activity and osteocalcin or perform Alizarin Red or Von Kossa stainings. You can check our papers if you want to have more info: Article Heparin-functionalized chitosan scaffolds for bone tissue engineering
andArticle Bone-Like Apatite-Coated Chitosan Scaffolds: Characterizatio...
. This paper is a good example on the use of these cells:Article PIASxβ is a key regulator of osterix transcriptional activit...
. I never worked with human osteoblastic cell lines, however, you may check this paper to have some ideas:Article A phenotypic comparison of osteoblast cell lines versus huma...
. I hope this info helps. Good luck with your experiments!
Rat BMSCs and whole blood can harvest from adult male Spraque-Dawly rats. Rat BMSCs were expanded in an osteogenic medium
We use primary human osteoblasts, isolated from surgery waste, and those cells in most cases outperform MSC regarding ALP activity and mineralization. It is also possible to buy human osteoblasts at different companies. A cheaper option would be to use the human osteosarcoma cell line SaOS-2, which is easy to handle and also shows high expression of osteogenic markers.
Hi Heba,
I worked on human mesenchymal stem cells derived from various tissues, like Warton’s jelly, adipose tissue and umbilical cord. In my experience they all can easily differentiate into osteoblast like cells, but I think among them the adipose tissue derived MSCs are the best choice. In comparison to bone marrow MSCs, you can have more access to their human source, simple extraction method with chemokines concentration gradient, also they're less sensitive to in vitro culture and expantion.
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