Can help with calculation of protein standard and dye for protein quantification assay(BCA)?

01 January 1970 1 10K Report

albumin concentration 2mg/ml and volume of vial 1 ml. I need five concentration to get curve. I don't know how much volume or concentration in each vial. Also I don't know how much buffer to add to the standard protein or my sample. Finally I don't know how much to add in 96 well played well.

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