What does the colors indicate in any heat map for miRNA profile. How to understand the Heatmap from just looking. Attached a picture from a paper.
I prepared manifest file using all FASTQ sequences and now I need to prepare metadata file. Is it a must? Also, how to convert manifest file (csv) into .qza My facility gave me a yml file with...
09 October 2019 6,446 1 View
My lab told me that the sequences are demultiplexed but the manifest file I generated from illuminating basespace have only sample ID and direction as in picture. How to know if data need...
09 October 2019 3,106 1 View
I am watching qiime importing metadata tutorial. I downloaded the barcode and sequence file using url and tried to open in qiime but they do not work as follow: (micro) a-PC:desktop hebahussein$...
09 October 2019 683 3 View
Hi. I activated qiime 2 2019, prepared a folder named '6 sample file' to try data import which has metadata, manifest, and 6 data sequences as in the picture. I received this error. maybe as...
09 October 2019 6,872 1 View
I got ethical approval for my research, but while submission, I noticed that editors comments need changing one word to the title into another word. Can I change it while I got ethical approval...
07 August 2018 3,891 9 View
is STROBE a reporting tool? so we need to make sure we reported every item of the checklist in our manuscript before submission? or we simply can mention that some items are not applicable to our...
07 August 2018 236 2 View
If I used same power, type 1 and 2 errors from a past similar research, what is the point of recalculating sample size? Can I just use same sample size with reference to the research? On the other...
07 August 2018 5,572 2 View
Or just one ethical approval.
07 August 2018 8,898 3 View
Hi. Any recommend youtube channel/website/ online course to understand the cycle of grant/budget planning/forming teams? Thanks
05 June 2018 2,495 0 View
any youtube channel/online courses/ website to help understand the cycle of grant application, budget planning, and team formation more than just simple google search? thanks
05 June 2018 3,468 1 View
Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...
01 March 2021 7,168 9 View
Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?
01 March 2021 9,785 3 View
I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...
01 March 2021 6,450 5 View
I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...
28 February 2021 8,626 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...
27 February 2021 5,681 3 View
Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...
26 February 2021 6,253 3 View
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
Need Help Does anyone Know how to make a Fractional impervious surface (FIS) map from NDVI map, in ArcGIS environment? Thanks in advance
24 February 2021 8,455 2 View