I would say it is possible. You might as well try it out since you have less alternative options

Yes you can strip it for few times. Try the low pH stripping buffer to strip your membrane for 30 min. It works really well.

The recipe is: 1.5g glycine, 0.1g SDS, 1ml Tween 20, add 100ml di water, pH 2.2.

Good luck

Hi!

You can do untill 10 times but you should to follow at least one rule: 1st staining by "pale" antibodies toprotein of low stability and the last by the most stable one (beta-tubulin, for example).

Regards,

Tatiana

Yes, you can. I have found the following protocol very helpful -http://www.abcam.com/ps/pdf/protocols/stripping%20for%20reprobing.pdf

A thing to consider is also whether you are reprobing for phosphorylated or total proteins. I always detect phospho proteins first followed by stripping and reprobing for total proteins. I have been unsuccesful in reprobing for phospho proteins. Also, PVDF membranes withstand the stripping reprobing better than nitrocellulose membrane. Hope this helps. Good luck.