I don't know the optimal antibody concentration so I may not reach good result and need to try another concentration (higher) using same membrane the next day so need to know how to store the membrane.
Dear Heba, you can but you don't have to strip a blot. Stripping will always loose in sensitivity. But you can check in a slightly different way. Make a SDS-PAGE with a larger well 1 to 2 cm or even larger, e.g. one well per gel). Add your sample (calculate/increase the sample size according to the width of your well), run the gel and transfer it as usual. After transfer cut the blot in small stripes as small as 1 to 2 mm is no problem or as small as you like. Store the stripes at -20. Proceed with the respective slice whenever you like by blocking, incubation with your abs. If you go as small as 1 mm, beware that the blot can turn around without your notice! So the band may occur at the opposite side. Good luck
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