Hi, if youre thinking of using beta mercaptoetanol as reducing agent it's not necessary because the ladder formula already has 50mM DTT.

Good luck!

Hi Heba,

The molecular weight maker is ready to use "as is", no need to add anything to it. Also, if you want to save, 5 ul is normally enough.

Hope this helps,

Ezequiel

Hello

Yes , you don't need to add anything .

http://www.bio-rad.com/en-au/applications-technologies/introduction-western-blot-protein-standards

Yes, no need to add anything. It is ready to used.

Bio-rad pre-stained markers are ready to use (as mentioned above), so we load the marker without adding anything to it.