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Questions related from Marine Ma
I recently performed a Western blot analysis using a sodium potassium ATPase antibody on HeLa cells. The results showed a band below 100 kD and a cluster between 100 and 150 kD. However,...
5 days ago 9,617 1 View
I am looking to convert the concentration of Human IFN-gamma protein from 1000 IU/ml to mg/ml. Could anyone provide guidance on the conversion process or share the resulting concentration? Your...
2 weeks ago 1,189 1 View
I am preparing to conduct in vivo animal studies with Ostamatinib (Cat. No. HY-13038A), administered via intraperitoneal injection. I have identified three different dissolution protocols using...
2 weeks ago 8,482 1 View
I am conducting Western blot analysis using the Phospho-IKB alpha (Ser32) antibody (YA183) on an HSF cell line. However, I am observing fuzzy bands across the entire membrane. What could be the...
2 weeks ago 385 1 View
I am working with debilitated mice and exploring the protective effects of carnosic acid on the kidneys and vascular endothelium in the context of sepsis-induced acute kidney injury, specifically...
2 weeks ago 4,511 1 View
I am interested in using Pronase E to digest and separate primary cells for my research. Has anyone successfully conducted this experiment? I would greatly appreciate it if you could share the...
2 weeks ago 2,801 1 View
I am preparing to use COL4A1 (HY-P71444) and COL4A2 (HY-P72147) in my experiments. Could anyone advise on the appropriate solvent or method for reconstituting these products? Your insights would...
3 weeks ago 3,019 2 View
I am in the process of formulating an oral dosage regimen for olorofim (HY-104029) that ensures safety, non-toxicity, and improved solubility. The intended dose is 16 mg/kg for an average animal...
3 weeks ago 9,488 4 View
Recently I bought some Cathepsin B protein (HY-P70830), I am looking for reliable methods to activate and measure the activity of Cathepsin B. If anyone has relevant experience or can point me to...
3 weeks ago 3,581 1 View
I am planning to dissolve Brequinar (HY-108325) at a concentration of 25 mg/mL in DMSO before mixing it with Aquaphor. Could anyone recommend an appropriate ultrasonic method for this dissolution...
3 weeks ago 1,199 1 View
I dissolved docosahexaenoic acid in ethanol, and tested it in DMEM full media at a concentration of 10 uM. However, I observed rounded shapes that I believe to be 'Lipid droplets'. Does anyone...
3 weeks ago 9,362 2 View
I have reviewed the product instructions for the Siglec-15 recombinant protein, which indicate that for short-term experiments (up to 7 days), the reconstituted protein solution can be stored at...
4 weeks ago 4,654 2 View
I am currently using the Mouse Neutrophil Negative Selection Kit (HY-K0350) to isolate neutrophils from C57BL/6 mice. I would like to inquire about the expected yield of neutrophils from both...
4 weeks ago 461 2 View
I am planning to dissolve Rotenone reagent (HY-B1756) in water for my experiments, but I am unsure about its solubility characteristics and concerned that it may be challenging to dissolve. Does...
4 weeks ago 4,803 3 View
Hydrocortisone (Cat. No. HY-N0583) is prepared at 10 mM per mL in DMSO. I would like to dilute it to 0.5 mg/mL for use in stem cell culture. How should I proceed? What diluent should I use, such...
4 weeks ago 4,504 3 View
How do I dissolve Donepezil? In water or DMSO?
4 weeks ago 3,691 3 View
I recently purchased BM-Cyclin (HY-K1059), which comes with Tiamulin fumarate and Minocycline hydrochloride packaged separately. Can I store them after reconstitution and mix them together?
5 weeks ago 1,728 1 View
Recently I bought Cardiotoxin Analog (CTX) IV (6-12) (No. HY-P1902), I would like to ask about its application in mouse model. 1. For solvent to dissolve CTX, is it okay to use 0.9% NaCl to...
5 weeks ago 9,490 1 View
When staining HepG2 cells with BODIPY 493/503 for lipid droplet detection, I notice the fluorescence signal is strong at first but fades within seconds under the green channel. What is the proper...
last month 1,923 2 View
I am conducting an experiment involving the intramuscular administration of KIN1408 dissolved in SBE-β-CD to 20 g female BALB/c mice. The doses being tested are 2 µg, 5 µg, 10 µg, 15 µg, and 20...
last month 6,107 2 View
Recently I bought Nab-Paclitaxel (HY-P99974) from MCE and it is intended for in vivo and in vitro experiments. The official website only provides reconstitution instructions: "The product can be...
last month 5,188 2 View
I am planning to intraperitoneally inject 30 mg/kg of Vofopitant dihydrochloride into mice weighing between 25-30 g. Could anyone provide guidance on the appropriate solvent formula for preparing...
last month 731 1 View
I have encountered a significant discrepancy between my Fluorescein Phalloidin staining results and those reported in the literature. Typically, Fluorescein Phalloidin staining produces...
last month 7,629 1 View
I am planning to use Hygromycin B (HY-B0490) as an antibiotic selection marker. However, the reconstitution instruction requires ultrasonic for both DMSO and water and we do not have a sonicator....
last month 5,732 2 View
I am looking to prepare a stock solution of Amilo 5Mer (TFA) for use in in vitro experiments with cells. Could anyone provide detailed instructions or best practices for preparing this solution?...
last month 4,390 1 View
I need to use JC-1 to stain RAW264.7 cells, but I only have JC-1 powder left in my lab. How should I dissolve it? Thanks in advance!
last month 3,722 2 View
I recently purchased some BODIPY-FL (HY-43520). Given the availability of numerous BODIPY-based lipid droplet dyes, I'm curious if I can use BODIPY-FL to stain lipid droplets in my samples. Can...
last month 5,497 1 View
If I want to administer 10 mg/kg of PKM2 inhibitor (HY-103617) via tail vein injection every 3 days for 8 weeks (12 animals per group, ~500 g each), which solvent formulation would be most suitable?
last month 2,488 2 View
I am currently preparing to administer Sorafenib to model mice weighing approximately 20 g, with a dosage of 30 mg/kg in a total volume of 200 μL via gavage. Could anyone provide guidance on the...
last month 6,747 1 View
I am seeking guidance on the specific dissolution protocol for administering RO8191 (HY-W063968) to model mice. The dosage is 20 mg/kg, and the intended dissolution regimen is 15% Solutol HS 15 +...
last month 1,355 1 View
Recently I bought SB431542 (HY-10431) and SRI-011381 (HY-100347), and I am planning to administer SB431542 at a dose of 10 mg/kg and SRI-011381 at 20 mg/kg to 20 g mice every two days. Could...
last month 213 1 View
How can I identify and validate high-affinity ligands for PHIP as potential warheads in PROTAC design, and is a workflow combining structure-based virtual screening with in vitro binding assays...
last month 6,429 2 View
I am looking to understand whether Isoflurane can be effectively used with anesthesia machines for administering inhalation general anesthesia in animal models. Are there specific considerations...
last month 8,434 1 View
Just went over the publication: PubMed: 39212900 and it says the study used both Tirzepatide(MedChemExpress, New Jersey, USA) and Retatrutide(MedChemExpress, New Jersey, USA). Is the TIZ used was...
last month 5,186 1 View
I am investigating the properties of ADAM-17 substrate (HY-P10174) and would like to know if they exhibit any background fluorescence. Additionally, what is the underlying principle behind their...
last month 7,345 2 View
We recently conducted an experiment using Adezmapimod (HY-10256) to investigate its effects on the phosphorylation of downstream proteins, specifically NF-κB and our target protein. However, we...
last month 5,528 1 View
I'm having an issue in Western blot with an ASC antibody(HY-P80548) as I can't detect any band. Experimental details: Raw264.7 macrophage cells, concentration around 3 μg/ml, sample volume 8-10...
04 August 2025 8,397 5 View
I'm investigating pharmacological inhibition of FTO using meclofenamic acid sodium (MA). Despite treating the cell for 24-48h across a broad concentration range (1-200 μM), we observe no...
04 August 2025 4,806 1 View
I am interested in using ER-Tracker Green for staining purposes and would like to know if it is suitable for use on fixed cells. Alternatively, can I fix the cells after staining with ER-Tracker...
01 August 2025 3,417 1 View
My recent Western blotting has been unsuccessful. I've been using the CDKN2A/p16INK4a Antibody (YA3494) and p21 Antibody, but the results always show multiple bands, and the bands appear faint and...
31 July 2025 5,151 1 View
I have observed that my cell lysates tend to be sticky during the lysis process. Is this a common occurrence? I'm using MCE NP-40 Lysis Buffer (HY-K1002). Any guidance or suggestions would be...
30 July 2025 2,417 1 View
I am experiencing an issue where my target protein is not detected in the extract from SH-SY5Y cells. I am wondering if this could be related to my A/G magnetic beads or another aspect of my...
29 July 2025 2,752 1 View
I am encountering an issue when using A/G magnetic beads for eluting membrane proteins from my samples. It seems that the proteins tend to aggregate during the elution process, which is hindering...
28 July 2025 1,601 1 View
I am working with GZMA/Granzyme A recombinant protein (HY-P76377 from MCE) in lyophilized powder form. After reconstituting it with 100 µL of ddH₂O and subsequently added 400µL of buffer...
25 July 2025 4,958 1 View
I am currently working on a project involving antibody-drug conjugates (ADCs) and encountered an issue with the adsorption efficiency of Protein A/G magnetic beads. After I injected mice with ADC,...
24 July 2025 6,662 1 View
I am currently investigating the effect of the Piezo1 agonist Yoda 1 (HY-18723, MCE) on calcium influx in endothelial cells. For my experiments, I incubated the cells with 2 μM Fluo-4 AM at 37°C...
23 July 2025 4,147 2 View
Recently I bought MitoTracker Deep Red and PicoGreen from MCE. I prepared MitoTracker Deep Red with 92 μL of DMSO to prepare a 1 mM stock solution, and stored it according to the COA conditions...
22 July 2025 8,186 1 View
I am seeking a reliable protocol for conjugating polyclonal antibodies to magnetic beads for the purpose of isolating proteins from serum. Given the wide variety of bead types available, which...
21 July 2025 2,329 1 View
I need to use human M-CSF(100ng/ml) to induce mononuclear cells differentiation into macrophage. But the issue is, should be use human serum or fetal bovine serum. Any suggestion would be greatly...
18 July 2025 7,715 2 View
First experiment: raw264.7 cells induced osteoclast differentiation, M-CSF (30 ng/ml) + RANKL (100 ng/ml), changed the medium every two days, no osteoclasts appeared on the 7th day of...
17 July 2025 1,772 1 View
Recently I bought some Thioflavin T (HY-D0218) from MCE. I'm curious that does Thioflavin T fluoresce when there is no amyloid aggregation? Does its fluorescence property depend solely on binding...
16 July 2025 6,502 1 View
I am currently doing CT imaging research on mouse brain tumors. The CT images produced by the laboratory before were difficult to observe the tumor boundary and growth status. If anyone has used...
15 July 2025 4,622 1 View
I just finished primary antibody staining and found that my secondary antibody was used up and I can't continue the experiment. If I soak the tissue in PBS, can I do secondary antibody staining...
14 July 2025 2,244 1 View
I am exploring the differences between EdU and CFSE in their applications for detecting cell proliferation. Can anyone shed light on the key distinctions between these two methods? Additionally,...
09 July 2025 9,062 1 View
I am interested in understanding the underlying principle of DND-189 staining. Could anyone provide insights into its mechanism of action or the specific applications for which it is most...
08 July 2025 7,838 1 View
I have a β-galactosidase staining kit (HY-K1089) that includes X-Gal, but I'm unable to use all of it before its expiration. What options do I have for preserving any unused X-Gal? Is it possible...
08 July 2025 7,589 1 View
I'm preparing PF-8380 (HY-13344 from MCE) for intraperitoneal administration in mice and considering PEG300 as the sole solvent, as I can dissolve PF-8380 smoothly with pure PEG300, but as soon as...
06 July 2025 2,633 1 View
I bought some lyophilized protein from MCE, and noticed that the instructions only mentioned protein should be 'diluted with the solution containing carrier proteins'. So are there any potential...
03 July 2025 8,474 1 View
I am looking for information on the protein labeling process for Sulfo-Cyanine7 (CY7, HY-D0825). Does anyone have insights or resources that could guide me through this procedure? Thanks in advance!
02 July 2025 937 2 View
I am curious whether Texas Red (HY-101878) experiences fluorescence quenching at 60°C. Has anyone conducted experiments or have insights into the stability of Texas Red under these conditions?
30 June 2025 4,791 3 View
I'm working with MCE Protein A/G magnetic beads (HY-K0202) where both antibody and antigen are currently bound. My goal is to recover only the antigen fraction while keeping the antibody coupled...
27 June 2025 7,467 4 View
Recently I bought BODIPY493/503 methyl bromide (HY-D1614) from MedChemexpress. Could anyone recommend the most suitable fluorescence channel to observe lipid droplets? Your insights would be...
26 June 2025 1,144 3 View
