I just finished primary antibody staining and found that my secondary antibody was used up and I can't continue the experiment. If I soak the tissue in PBS, can I do secondary antibody staining after a few days? Then when I do secondary antibody staining again, do I need to use primary antibody to stain the tissue again? Or does this method not work? Thanks in advance!
Hi, Marine Ma . It depends on the expression level and stability of the detected antigen, as well as the quality of the primary antibody.
Previously, we conducted an experiment where samples were soaked in PBS overnight after incubating with the primary antibody. For certain antigens, this soaking had no effect, while for others, it did. However, soaking in PBS overnight may lead to increased background staining in subsequent steps.
If the tissue samples are precious, you can try re-incubating with the primary and secondary antibodies. If the staining results are still affected, it may be necessary to obtain new samples and repeat the staining process.
The answer to this question comes from MedChemExpress (MCE) Technical Support. [email protected]
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