Hi，

Possible reasons:

1) The PVDF/NC membrane is contaminated or the membrane surface is touched during transfer;

(2) The blocking solution is contaminated or contains insoluble particles;

(3) The membrane is not fully activated or the membrane becomes dry after activation;

Improvement methods:

(1) Use tweezers to hold a corner during transfer and do not touch the membrane surface;

(2) Replace the blocking solution and clean the blocking box to avoid insoluble particles or damage to the membrane surface;

(3) Ensure that the membrane is fully activated and immersed in the transfer solution to keep it moist;

You can try adjusting the primary antibody dilution ratio to 1:1000.

The answer to this question is provided by MedChemExpress Technical Support.

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