The calculated working solution concentration is 3 mg/mL. Due to the relatively weak constitution of the model mice, it is recommended to reduce the DMSO concentration to 2%.

Preferred Dissolution Scheme: 2% DMSO + 40% PEG300 + 5% Tween-80 + 53% saline.

• First, prepare a 150 mg/mL stock solution in DMSO.
• For 1 mL of working solution:Add 20 μL of the clear DMSO stock solution to 400 μL of PEG300 and mix thoroughly. Add 50 μL of Tween-80 to the mixture above and mix thoroughly. Adjust the final volume to 1 mL by adding 530 μL of saline.
• To account for experimental losses during the procedure, prepare sufficient volume for one additional animal. The answer to this question comes from MedChemExpress Technical Support.
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