Hello Marine Ma

For in vitro experiment:

For preparing 1mL of 1µM working solution, the calculation is provided below.

Let us assume you have a stock solution of 5mg/mL nab-paclitaxel.

You will have to calculate the molarity of your stock solution.

First, convert 5 mg/mL to g/L:

So, 5 mg/mL = 5 g/L

Now, Moles in stock = 5 g/L / 853.9 g/mol = 0.00585 mol/L, where Mol. wt. of nab-paclitaxel is 853.9g/mol.

Next, you calculate the amount of nab-paclitaxel needed (moles):

Moles needed = 1 x 10⁻⁶ M * 0.001 L = 1 x 10⁻⁹ moles

Finally, calculate the volume of stock needed:

Volume of Stock = 1 x 10⁻⁹ moles / 0.00585 mol/L = 0.000171 L = 171 µL.

Similarly calculate for 10µM , 30µM, and 50µM concentrations.

For in vivo experiment:

Let us suppose for in vivo experiment, the weight of the mouse is 20gm. Therefore, to dose at 20 mg/kg, you need to divide your dose by 50 which means your 20 g mouse should receive 0.4mg (20/50). You should prepare 4mg/ml stock solution. Then to dose at 20 mg/kg, you would need 100uL which would contain 0.4 mg.

Best,

This product contains excipients. You can try to dissolve it using sterile PBS or saline. The concentration after dissolution can be approximately 5 mg of paclitaxel per milliliter. Please note that the resulting solution will be a homogeneous suspension, not a clear liquid. This product is unstable and it is recommended to prepare and use it immediately.

The answer to this question is provided by MedChemExpress Technical [email protected]