Hello Marine Ma

For general lipid droplet imaging with BODIPY 493/503, the green channel is the standard and most reliable choice. BODIPY 493/503 exhibits a bright green fluorescence when it binds to neutral lipids, making it ideal for visualizing lipid droplets in cells.

FYI: BODIPY dyes, like BODIPY 493/503 and BODIPY 505/515, are excited by blue light, typically in the range of 460-490 nm. When excited, BODIPY dyes emit a green fluorescence. For BODIPY 493/503 and 505/515, the emission peak is around 515-530 nm. Some may suggests BODIPY dyes can exhibit red fluorescence in certain situations, but that is typically when using specific excitation wavelengths or when considering factors like photostability and Stokes shifts.

You may want to refer to the article attached below for more information.

Article BODIPY‐based Fluorescent Indicators for Lipid Droplets

Best,

BODIPY493/503 methyl bromide has an emission wavelength of 503 nm, so the FITC channel can be used for detection. The answer to this question comes from MCE Technical Support.

[email protected] Lucian Miles

Thanks Malcolm Nobre , Qiu Weiwei !