We recently conducted an experiment using Adezmapimod (HY-10256) to investigate its effects on the phosphorylation of downstream proteins, specifically NF-κB and our target protein. However, we observed no significant differences in phosphorylation levels following treatment.
Could anyone provide insights into potential reasons for this lack of response? Are there specific factors or experimental conditions we should consider that might influence the phosphorylation outcomes? Your expertise and suggestions would be greatly appreciated!
Hi Marine, I could not find any literature evidence showing that this product can reliably detect NF-κB phosphorylation. I would suggest using a suitable antibody to detect the downstream target HSP27 instead.
The answer to this question comes from MCE Technical Support.
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