Hello Marine Ma,
Dissolving donepezil depends on whether you have the hydrochloride salt form or the freebase form. So, always confirm whether you have the hydrochloride salt or the freebase. This is the most critical factor for determining the correct solvent. The salt form will typically be specified on the product label. Donepezil hydrochloride is highly soluble in water, while donepezil freebase is poorly soluble in water and requires organic solvents like DMSO.
To dissolve donepezil hydrochloride add the powder to water. Gentle sonication or warming to 37°C can help speed up the process if needed, but it is typically not required for concentrations up to 20 mg/ml. For cell-based assays, if donepezil freebase is used, DMSO is commonly used to create a stock solution, which is then diluted using an aqueous buffer or culture media.
Best,
Previously, we tested the solubility of HY-14566 and found it insoluble in water. It is recommended to prepare the stock solution using pure DMSO, preferably cell culture grade suitable for cell cryopreservation. Before dosing cells, dilute the stock 1000-fold with culture medium to the working concentration. If precipitation occurs during dissolution, ultrasonic cleaning at 37°C with a sonicator can be used to assist dissolution.
The answer to this question is provided by MedChemExpress Technical Support. [email protected]
DMSO is a very good solvent for preparation of stock concentration because it dissolves most chemical classes. Once the stock is prepared with DMSO, the concentration of this solvent can be brought below toxic levels during preparation of test concentration from the stock by diluting with reagent used to run assay.
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