Recently I bought Cardiotoxin Analog (CTX) IV (6-12) (No. HY-P1902), I would like to ask about its application in mouse model.
1. For solvent to dissolve CTX, is it okay to use 0.9% NaCl to dissolve it as stock solution? What concentration is recommended to use as stock solution?
2. I plan to inject 50 uL CTX into TA muscle of mouse, what concentration is recommended to use as working solution? Some papers use 10 mM, while others use 10 uM.
3. How long is enough to induce muscle degeneration? Following this, I will administer another drug to mouse to see the treat efficiency of that drug. Some papers showed they harvested muscles after 48 hours. Does that mean I could not inject target drug until 48 hours after CTX administration?
I really appreciate any support and thank you on advance!
Hi Marine Ma
You can refer to the relevant literature on CTX used to induce muscle damage (e.g. PMID: 33997104; PMID: 22490927). It is recommended that you find more literature later and set high and low doses to do a preliminary experiment.
The maximum solubility of HY-P1902 in PBS is 50 mg/mL. It must be prepared immediately for use in experiments and cannot be stored.
If your working solution concentration is lower in subsequent experiments, consider preparing a higher-concentration stock solution in ddH2O and then diluting it to the working solution concentration in PBS before subsequent experiments. This diluted working solution must be prepared immediately for use and cannot be stored.
The answer to this question is provided by MedChemExpress Technical Support.