I have a β-galactosidase staining kit (HY-K1089) that includes X-Gal, but I'm unable to use all of it before its expiration. What options do I have for preserving any unused X-Gal? Is it possible to repackage or store it in a way that maintains its effectiveness? Thanks in advance!
Marine Ma ,
Yes, It can be aliquoted.
The β-galactosidase staining kit (HY-K1089) provided by MedChemExpress (MCE) is based on the principle that SA-β-gal catalyzes X-Gal to produce a blue product at pH 6.0, so that the aging of cells or tissues can be observed under an optical microscope.
Since X-Gal solution will freeze when stored at 20℃ or 4℃, it can be completely thawed by placing it in a water bath at room temperature or 37℃ for 2-5 minutes and shaking it appropriately. If you need to aliquot X-Gal solution or prepare staining working solution, use polypropylene (PP) or glass consumables, and avoid using polystyrene (PS) consumables (such as cell culture plates, serological pipettes, etc.).
The answer to this question comes from MCE Technical Support.
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