I dissolved docosahexaenoic acid in ethanol, and tested it in DMEM full media at a concentration of 10 uM. However, I observed rounded shapes that I believe to be 'Lipid droplets'. Does anyone know if this is a normal reaction or oxidation/contamination? Also, when I mix DHA dissolved in ethanol into the medium, the medium becomes slightly cloudy, and I wonder if this is normal dissolution. Any insights or recommendations based on your experience would be greatly appreciated!
From your description, it seems that docosahexaenoic acid is not completely dissolved. Since the compound itself is a fatty acid, after the product is dissolved in ethanol, it is recommended to assist with ultrasonic heating to dissolve. When no "fat droplets" can be seen under the microscope, it can be considered to be completely dissolved. After dilution into the culture medium, turbidity appears, which means that it is not dissolved and there is precipitation. It can also be assisted with ultrasonic heating to dissolve. After it is completely dissolved, change the solution and add it to the cells.
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Marine Ma
Docosahexaenoic acid can react with DMEM medium. DMEM contains all amphoteric amino acids and amphoteric phenol red. The latter compounds, depending on pH, can have positive charges that react with the negative charge of the carboxyl group of the acid.
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