I am interested in understanding the underlying principle of DND-189 staining. Could anyone provide insights into its mechanism of action or the specific applications for which it is most effective? Any detailed explanations or references would be greatly appreciated!
DND-189 (HY-D1457, MCE) is a pH-sensitive probe, and its fluorescence characteristics change with the pH value of the environment:
almost no fluorescence is produced in a neutral or weakly alkaline environment, while in an acidic environment (pH < 5.5), the fluorescence intensity is significantly enhanced.
The pH-sensitive properties of DND-189 match the acidic microenvironment of lysosomes, and specifically produce detectable fluorescence signals in the lysosomal region, so it can be used to label lysosomes.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
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