Are there any Experience with Using Pronase E for Digesting and Isolating Primary Cells?

last month 1 3K Report

I am interested in using Pronase E to digest and separate primary cells for my research. Has anyone successfully conducted this experiment? I would greatly appreciate it if you could share the specific experimental steps or protocol you followed. Any tips or insights would also be helpful!

Jenny Hou

For the isolation of primary cells using Pronase E digestion, we highly recommend the more active form Pronase E (Activity ≥ 4000 U/mg) (HY-114158A) for improved cell viability. A detailed protocol for its application can be found in the following reference: PMID: 36192599. Additionally, specific dosage information is available in this study: PMID: 36994456.

The answer to this question is provided by MedChemExpress Technical [email protected]

Badges
Science topic

More Marine Ma's questions See All

How can we improve storage conditions of museum objects?

90% of museum objects worldwide are in storage.

21 July 2024 8,038 6 View

How to removal of dead cells and debris from organoid cultures?

I'm currently working with human pancreatic organoids and I am wondering to remove the dead cells and debris from health organoids, any suggestions would be appreciated!

17 July 2024 8,330 1 View

Does it work as antibiotic resistance if plamid has no RBS??

I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...

11 July 2024 7,847 1 View

Can a liquid metal-ligand sample be directly analyze in flame atomic absorption spectrosocopy?

Can I directly analyze a liquid metal-ligand sample without acid digestion in flame atomic absorption spectroscopy?

25 June 2024 1,866 2 View

Is this a mixed methods approach?

For my PhD dissertation: If Chapter 1 is a systematic literature review (QUANTS/QUALS) while Chapters 2, 3, and 4 are empirical chapters (QUALS), is this considered a mixed methods research...

16 June 2024 3,220 5 View

What can I do if primer solution gives band in electrophoresis before PCR reaction?

Trouble 1: I ran a PCR reaction and found a low molecular weight band (100 bp) when doing electrophoresis. 1ng plasmid (OD260/280=1.84) was used as template. Final concentration of primers was...

13 June 2024 5,540 6 View

How to find the original questionnaire of the article-Dimensional analysis of schoolchildren’s food label comprehension: a pilot study?

27 May 2024 2,551 0 View

Why the Mixed mode simulation always exit with status 0?

I encountered some issues while simulating the transient characteristics of a device using Mixed Mode. After executing the following codes, an error message "Simulator has unexpectedly exited with...

23 May 2024 2,238 0 View

Can bands of cell lysate be seen during SDS-PAGE?

I want to detect my target protein through WB and it worked well before. But now I could not get any signal for both my target and the GAPDH, so I ran SDS-PAGE. I expected many bands on the gel of...

23 May 2024 613 6 View

Can I ask for a JCPDS card no. 01-083-1436 or CPDS file number 00-031-1979 for urea?

Can I ask for a JCPDS card no. 01-083-1436 or CPDS file number 00-031-1979? I need to index a urea derivative compound to the face-centered tetragonal structure, JCPDS card no....

15 May 2024 9,751 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Is it necessary to autoclave every vessel used in DNA isolation?

DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?

10 July 2024 7,526 3 View

What are the common mistakes occur during DNA isolation in plants?

General mistakes which could happen during DNA isolation of plants

10 July 2024 9,488 3 View