Are there any Experience with Using Pronase E for Digesting and Isolating Primary Cells?

2 weeks ago 1 3K Report

I am interested in using Pronase E to digest and separate primary cells for my research. Has anyone successfully conducted this experiment? I would greatly appreciate it if you could share the specific experimental steps or protocol you followed. Any tips or insights would also be helpful!

Jenny Hou

For the isolation of primary cells using Pronase E digestion, we highly recommend the more active form Pronase E (Activity ≥ 4000 U/mg) (HY-114158A) for improved cell viability. A detailed protocol for its application can be found in the following reference: PMID: 36192599. Additionally, specific dosage information is available in this study: PMID: 36994456.

The answer to this question is provided by MedChemExpress Technical [email protected]

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