I bought some lyophilized protein from MCE, and noticed that the instructions only mentioned protein should be 'diluted with the solution containing carrier proteins'. So are there any potential limitations regarding the composition of the buffer used, and any specific factors or components should be avoided to ensure optimal protein stability and activity? Thanks in advance!
The commonly used carrier protein-containing buffer for recombinant protein reconstitution typically employs PBS or basic culture medium as the base system, supplemented with a defined concentration of carrier proteins to maintain protein stability. Commonly used carrier proteins include 0.1% BSA (bovine serum albumin), 5% HSA (human serum albumin), 10% FBS (fetal bovine serum), or 5% trehalose. These components effectively prevent protein adsorption, aggregation, and concentration loss, thereby preserving the activity and stability of the recombinant protein. MCE provides related products: BSA (HY-D0842), HSA (HY-P1956), trehalose (HY-CP001), and FBS (HY-T1000). For further technical inquiries, please feel free to contact us.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
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