I need to use JC-1 to stain RAW264.7 cells, but I only have JC-1 powder left in my lab. How should I dissolve it? Thanks in advance!
Lily Watson
First, centrifuge the sample. Then dissolve it in a certain volume of DMSO. To aid dissolution, ultrasonic heating at 37°C can be applied. This yields a 200 μM JC-1 stock solution in DMSO. Aliquot and store the stock at -20°C or -80°C, protecting it from light.
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Amin Aghaee Badelbuo
Solvent: Use high‑purity DMSO (dimethyl sulfoxide) as the primary solvent. Concentration: Prepare a concentrated stock (e.g., 1–2 mM) to minimize freeze‑thaw cycles. Procedure:Warm the vial to room temperature before opening to avoid condensation. Add the required volume of anhydrous DMSO. Vortex gently or pipette up and down until fully dissolved. Storage:Aliquot into small volumes. Store at −20 °C, protected from light (wrap in foil or use amber tubes). Working Solution: Dilute the stock in the appropriate cell culture buffer/medium just before use.
JC‑1 (5,5′,6,6′‑tetrachloro‑1,1′,3,3′‑tetraethyl‑benzimidazolylcarbocyanine iodide) is a cationic, lipophilic dye commonly used for mitochondrial membrane potential assays.
To dissolve JC‑1 powder properly:
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